NGS- und PCR-Cleanup .
Magnetic Beads sind sehr effizient bei der Aufreinigung von PCR-Produkten und der Größenselektion von Amplikons bei der Sequenzierung.
Unsere MagSi-NGSPREP Plus Beads können direkt in bereits etablierten NGS-Protokollen genutzt werden, z.B. anstelle von Ampure XP Beads.
Hard Facts .
- Hervorragende Aufreinigung von PCR-Produkten
- Effiziente Entfernung von Enzymen, Primern, Oligonukleotiden, Polymerasen und anderen Verunreinigungen
- Hohe Reinheit und Recovery, konsistente Sequenzierergebnisse
- Einfach skalierbar und HTS-kompatibel
- SPRI-basierte Size-Selection
- Optimiert für Biomek® Workstations und Hamilton® Microlab STARline
- Kompatibel mit vielen anderen automatisierten Liquid-Handling-Systemen (z.B. PerkinElmer®, Caliper Life Sciences®, etc.)
Anwendungen .
Die library preparation ist einer der wichtigsten Schritte in der Probenvorbereitung für NGS Reaktionen. MagSi NGS Beads ermöglichen eine einfache Größenselektion durch die Variation des Bead : Probe-Verhältnisses.
Die MagSi-NGSPREP Plus Beads sind auch geeignet für eine Größenselelektion Ihrer PCR-Produkte (SPRI).
Durch Variation des Beads:Sample-Verhältnisses können Sie gezielt erwünschte Fragmentgrößen selektieren bzw. unerwünschte Fragmentgrößen entfernen.
Mit den MagSi-NGSPREP Plus Beads entfernen Sie Enzyme, Primer, Oligonukleotide, sowie Polymerasen und Salze einfach und schnell aus Ihrem PCR-Produkt.
Mit einem angepassten Protokoll können die MagSi-NGSPREP Plus Beads auch zur Anreicherung von RNA verwendet werden, um die Sensitiviät und Spezifität der Reversen Transription und qPCR-Analyse zu verbessern.
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NGSPrep Plus Beads .
Downloads .
Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016–2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108
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