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Steinbrenner Laborsysteme GmbH

In der Au 17, 69257 Wiesenbach

+49 (0) 6223 / 96730-0

mail@steinbrenner.de

Laborprodukte: Magnetic Beads

NGS- und PCR-Cleanup .

Magnetic Beads sind sehr effizient bei der Aufreinigung von PCR-Produkten und der Größenselektion von Amplikons bei der Sequenzierung.

Unsere MagSi-NGSPREP Plus Beads können direkt in bereits etablierten NGS-Protokollen genutzt werden, z.B. anstelle von Ampure XP Beads.

Hard Facts .

Anwendungen .

Die library preparation ist einer der wichtigsten Schritte in der Probenvorbereitung für NGS Reaktionen. MagSi NGS Beads ermöglichen eine einfache Größenselektion durch die Variation des Bead : Probe-Verhältnisses.

Die MagSi-NGSPREP Plus Beads sind auch geeignet für eine Größenselelektion Ihrer PCR-Produkte (SPRI).

Durch Variation des Beads:Sample-Verhältnisses können Sie gezielt erwünschte Fragmentgrößen selektieren bzw. unerwünschte Fragmentgrößen entfernen.

Mit den MagSi-NGSPREP Plus Beads entfernen Sie Enzyme, Primer, Oligonukleotide, sowie Polymerasen und Salze einfach und schnell aus Ihrem PCR-Produkt.

Mit einem angepassten Protokoll können die MagSi-NGSPREP Plus Beads auch zur Anreicherung von RNA verwendet werden, um die Sensitiviät und Spezifität der Reversen Transription und qPCR-Analyse zu verbessern.

Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016–2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108

Downloads .

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MagSi-NGSprep Plus – Produktdatenblatt 598.40 KB

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MagSi-NGSPREP Plus – Manual 557.09 KB

Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016–2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108

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Pannekens, M. et al (2020). Densely Populated Water Droplets in Heavy-Oil Seeps. Applied and Environmental Microbiology 86(11). https://doi.org/10.1128/AEM.00164-20

Goldmann, K. et al (2020). Early Stage Root-Associated Fungi Show a High Temporal Turnover, but Are Independent of Beech Progeny. Microorganisms 8(2). https://doi.org/10.3390/microorganisms8020210

Schmitt, F.C.F. et al (2020). Pulmonary microbiome patterns correlate with the course of disease in patients with sepsis-induced ARDS following major abdominal surgery. Journal of Hospital Infection 105(3). https://doi.org/10.1016/j.jhin.2020.04.028

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Ahle, C.M. et al (2020). Staphylococcus saccharolyticus: An Overlooked Human Skin Colonizer. Microorganisms 8(8). https://doi.org/10.3390/microorganisms8081105

Kressler, C. et al (2021). Targeted De-Methylation of the FOXP3-TSDR Is Sufficient to Induce Physiological FOXP3 Expression but Not a Functional Treg Phenotype. Frontiers in Immunology 11. https://doi.org/10.3389/fimmu.2020.609891

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