NGS and PCR cleanup .

Magnetic beads are very efficient for the purification of PCR products and size selection of amplicons in sequencing.

Our MagSi-NGSPREP Plus beads are comptible with most established NGS protocols, e.g. Ampure XP .

Hard Facts .

Excellent purification of PCR products
Efficient removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
High purity and recovery, consistent sequencing results
Easily scalable and HTS compatible
SPRI-based size selection
Optimized for Biomek® Workstations and Hamilton® Microlab STARline
Compatible with many other automated liquid handling systems (e.g. PerkinElmer®, Caliper Life Sciences®, etc.)

Applications .

Library preparation is one of the most important steps in sample preparation for NGS reactions. MagSi NGS beads allow easy size selection by varying the bead : sample ratio.

Size selection of PCR products

The MagSi-NGSPREP Plus beads are also suitable for size selection of your PCR products (SPRI).

By varying the bead-to-sample ratio, you can select desired fragment sizes or remove unwanted fragment sizes.

Purification of PCR products

With MagSi-NGSPREP Plus Beads you can remove enzymes, primers, oligonucleotides, as well as polymerases and salts easily and quickly from your PCR product.

RNA enrichment

With an adapted protocol, MagSi-NGSPREP Plus beads can also be used to enrich RNA to improve the sensitivity and specificity of reverse transcription and qPCR analysis.

Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016-2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108