NGS and PCR cleanup .
Magnetic beads are very efficient for the purification of PCR products and size selection of amplicons in sequencing.
Our MagSi-NGSPREP Plus beads are comptible with most established NGS protocols, e.g. Ampure XP .
Hard Facts .
- Excellent purification of PCR products
- Efficient removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
- High purity and recovery, consistent sequencing results
- Easily scalable and HTS compatible
- SPRI-based size selection
- Optimized for Biomek® Workstations and Hamilton® Microlab STARline
- Compatible with many other automated liquid handling systems (e.g. PerkinElmer®, Caliper Life Sciences®, etc.)
Applications .
Library preparation is one of the most important steps in sample preparation for NGS reactions. MagSi NGS beads allow easy size selection by varying the bead : Probe ratio.
The MagSi-NGSPREP Plus beads are also suitable for size selection of your PCR products (SPRI).
By varying the bead-to-sample ratio, you can select desired fragment sizes or remove unwanted fragment sizes.
With MagSi-NGSPREP Plus Beads you can remove enzymes, primers, oligonucleotides, as well as polymerases and salts easily and quickly from your PCR product.
With an adapted protocol, MagSi-NGSPREP Plus beads can also be used to enrich RNA to improve the sensitivity and specificity of reverse transcription and qPCR analysis.
Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016-2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108
NGSPrep Plus Beads .
Downloads .
Publications
Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016-2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108
Kaupper, T. et al (2021). Recovery of Methanotrophic Activity Is Not Reflected in the Methane-Driven Interaction Network after Peat Mining. Applied and Environmental Microbiology 87(5). DOI: https://doi.org/10.1128/AEM.02355-20.
Zhan, T. et al (2020). Multi-omics integration identifies a selective vulnerability of colorectal cancer subtypes to YM155. International Journal of Cancer 148(8). https://doi.org/10.1002/ijc.33393
Steudel, B. et al (2021). Microeukaryotic Communities on the Fruit of Gardenia thunbergia Thunb. with a Focus on Pathogenic Fungi. Pathogens 10(5), 555. https://doi.org/10.3390/pathogens10050555
Kaupper, T. et al (2021). When the going gets tough: Emergence of a complex methane-driven interaction network during recovery from desiccation-rewetting. Soil Biology and Biochemistry 153. https://doi.org/10.1016/j.soilbio.2020.108109
Flasbeck, V.; Brüne, M. (2021). Association between childhood maltreatment, psychopathology and DNA methylation of genes involved in stress regulation: evidence from a study in Borderline Personality Disorder. PLos One. https://doi.org/10.1371/journal.pone.0248514
Spanker, D.-T.; Reuscher, C. M.; Klug, G. (2021). Impact of PNPase on the transcriptome of Rhodobacter sphaeroides and its cooperation with RNase III and RNase E . BMC Genomics 22(106). https://doi.org/10.1186/s12864-021-07409-4
Heise, J.; Müller, H.; Probst, A. J.; Meckenstock, R. U. (2021). Ammonium Removal in Aquaponics Indicates Participation of Comammox Nitrospira. Current Microbiology 78. https://doi.org/10.1007/s00284-021-02358-3
Fan, L. et al (2021). Active metabolic pathways of anaerobic methane oxidation in paddy soils. Soil and Soil Biochemistry 156. https://doi.org/10.1016/j.soilbio.2021.108215
Black S. R. et al (2021). Limited antimicrobial efficacy of oral care antiseptics in microcosm biofilms and phenotypic adaptation of bacteria upon repeated exposure. Clinical Oral Investigations 25. https://doi.org/10.1007/s00784-020-03613-w
Basic, M. et al (2021). Monitoring and contamination incidence of gnotobiotic experiments performed in microisolator cages. International Journal of Medical Microbiology 311(3). https://doi.org/10.1016/j.ijmm.2021.151482
Hegyi, A.; Nguyen, T. B. K.; Posta, K. (2021). Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria. Microorganisms 9(9). https://doi.org/10.3390/microorganisms9091796
Mayer, Z. et al (2021). Impact of Soil-Applied Microbial Inoculant and Fertilizer on Fungal and Bacterial Communities in the Rhizosphere of Robinia sp. and Populus sp. Plantations. Forests 12(9). https://doi.org/10.3390/f12091218
Sommer, A. et al (2021). Induced systemic resistance impacts the phyllosphere microbiome through plant-microbe-microbe interactions. bioRxiv. https://doi.org/10.1101/2021.01.13.426583
Wallen-Russell, C.; Gijsberts-Veens, A.; Wallen-Russell, S. (2021). Could Modifying the Skin Microbiome, Diet, and Lifestyle Help with the Adverse Skin Effects after Stopping Long-Term Topical Steroid Use? Allergies 2(1). https://doi.org/10.3390/allergies2010001
Huesa, J. et al (2021). Structure-based analyses of Salmonella RcsB variants unravel new features of the Rcs regulon. Nucleic Acids Research 49(4). https://doi.org/10.1093/nar/gkab060
Rosenau S et al (2021). The Effect of a Total Fishmeal Replacement by Arthrospira platensis on the Microbiome of African Catfish (Clarias gariepinus). Life 11(6). https://doi.org/10.3390/life11060558
Pannekens, M. et al (2020). Densely Populated Water Droplets in Heavy-Oil Seeps. Applied and Environmental Microbiology 86(11). https://doi.org/10.1128/AEM.00164-20
Goldmann, K. et al (2020). Early Stage Root-Associated Fungi Show a High Temporal Turnover, but Are Independent of Beech Progeny. Microorganisms 8(2). https://doi.org/10.3390/microorganisms8020210
Schmitt, F.C.F. et al (2020). Pulmonary microbiome patterns correlate with the course of disease in patients with sepsis-induced ARDS following major abdominal surgery. Journal of Hospital Infection 105(3). https://doi.org/10.1016/j.jhin.2020.04.028
Wiper, D. et al (2020). Novel Antimicrobial Cellulose Fleece Inhibits Growth of Human-Derived Biofilm-Forming Staphylococci During the SIRIUS19 Simulated Space Mission. Frontiers in Microbiology 11. https://doi.org/10.3389/fmicb.2020.01626
Ahle, C.M. et al (2020). Staphylococcus saccharolyticus: An Overlooked Human Skin Colonizer. Microorganisms 8(8). https://doi.org/10.3390/microorganisms8081105
Kressler, C. et al (2021). Targeted De-Methylation of the FOXP3-TSDR Is Sufficient to Induce Physiological FOXP3 Expression but Not a Functional Treg Phenotype. Frontiers in Immunology 11. https://doi.org/10.3389/fimmu.2020.609891
Ricci, C. et al (2019). PD-1 (PDCD1) promoter methylation in Merkel cell carcinoma: prognostic relevance and relationship with clinico-pathological parameters. Modern Pathology 32. https://doi.org/10.1038/s41379-019-0261-5
Moser, D. A. et al (2020). Targeted bisulfite sequencing: A novel tool for the assessment of DNA methylation with high sensitivity and increased coverage. Psychoneuroendocrinology 120. https://doi.org/10.1016/j.psyneuen.2020.104784
Liang, S. et al (2020). Transcriptional mutagenesis dramatically alters genome-wide p53 transactivation landscape. Scientific reports (10(1). https://doi.org/10.1038/s41598-020-70412-4
Gerovac, M. et al (2020). Global discovery of bacterial RNA-binding proteins by RNase-sensitive gradient profiles reports a new FinO domain protein. RNA 26(10). https://doi.org/10.1261/rna.076992.120
Fussmann, D. et al (2020). Authigenic formation of Ca-Mg carbonates in the shallow alkaline Lake Neusiedl, Austria. Biogeosciences 17. https://doi.org/10.5194/bg-17-2085-2020
Fuchs, M. et al (2020). An RNA-centric global view of Clostridioides difficile reveals broad activity of Hfq in a clinically important Gram-positive bacterium. bioRxiv. https://doi.org/10.1101/2020.08.10.244764
Renzi, A. et al (2019). Prevalence of p53 dysregulations in feline oral squamous cell carcinoma and non-neoplastic oral mucosa. PLoS One 14(4). https://doi.org/10.1371/journal.pone.0215621
Lüneberg, K. et al (2019). Land Use Change and Water Quality Use for Irrigation Alters Drylands Soil Fungal Community in the Mezquital Valley, Mexico. Frontiers in microbiology 10. https://doi.org/10.3389/fmicb.2019.01220