NGS- und PCR-Cleanup .
Magnetic Beads sind sehr effizient bei der Aufreinigung von PCR-Produkten und der Größenselektion von Amplikons bei der Sequenzierung.
Unsere MagSi-NGSPREP Plus Beads können direkt in bereits etablierten NGS-Protokollen genutzt werden, z.B. anstelle von Ampure XP Beads.
Mit den MagSi-DT Removal Beads lassen sich Dye-Terminatoren und nicht inkorporierte Farbstoffe aus Sequenzierungsreaktionen entfernen – ohne Zentrifugation oder Filtration .
Hard Facts .
- Kompatibel mit existierenden Protokollen
- Hohe Reinheit und Recovery
- Einfach skalierbar und HTS-kompatibel
- MagSi DT-Removal: Entfernung von Verunreinigungen wie Dye-Terminatoren
- MagSi-NGSPREPplus: SPRI-basierte Size-Selection
- Garantieren konsistente und robuste Ergebnisse
Anwendungen .
Die library preparation ist einer der wichtigsten Schritte in der Probenvorbereitung für NGS Reaktionen. MagSi NGS Beads ermöglichen eine einfache Größenselektion durch die Variation des Bead : Probe-Verhältnisses.
Die MagSi-NGSPREP Plus Beads sind auch geeignet für eine Größenselelektion Ihrer PCR-Produkte (SPRI).
Durch Variation des Beads:Sample-Verhältnisses können Sie gezielt erwünschte Fragmentgrößen selektieren bzw. unerwünschte Fragmentgrößen entfernen.
Mit den MagSi-NGSPREP Plus Beads entfernen Sie Enzyme, Primer, Oligonukleotide, sowie Polymerasen und Salze einfach und schnell aus Ihrem PCR-Produkt.
Entfernen von nicht inkorporierten Farbstoffen und Salzen nach Sequenzierungs-Reaktionen mit unseren MagSi-DT Removal Beads.
Mit einem angepassten Protokoll können die MagSi-NGSPREP Plus Beads auch zur Anreicherung von RNA verwendet werden, um die Sensitiviät und Spezifität der Reversen Transription und qPCR-Analyse zu verbessern.
Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016–2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108
Details .
Easy-to-use .
- MagSi-NGSPREP: Ein Produkt für all Cleanup- und Größenselektions-Schritte im library preparation Workflow
- Unkompliziertes Protokoll mit Bind-Wasch-Elute-Verfahren
- Für manuelle und automatisierte Workflows
Downloads .
Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016–2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108
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