dPCR for Digital LightCycler® .

The PLEXUS dPCR Master Mix for Digital LightCycler Systems® offers reliable, high-precision quantification of DNA. The optimized formulation enables stable and efficient amplification in the nanoplate partitions and provides reproducible results across a broad range of targets and assay designs. This makes the PLEXUS dPCR Master Mix ideal for applications such as absolute quantification, rare variants or copy number analysis.

Thanks to its robust reaction chemistry, the PLEXUS dPCR Master Mix offers high signal strength with low background and supports precise cluster separation for reliable analysis. The formulation is fully compatible with Digital LightCycler Nanoplates and common hydrolysis-based probe assays, ensuring seamless integration into existing workflows.

Key Facts .

For Roche Digital LightCycler® systems
For all kinds of probes (e.g. TaqMan®)
Suitable for multiplexing
Ready-to-use
Stable at room temperature - pipetting without ice
Developed & manufactured in Germany

Applications .

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Absolute quantification

Digital PCR enables the direct determination of the absolute copy number of DNA or RNA targets without a standard curve. This means that even small differences in the target molecule concentration can be reliably measured.

Detection of rare mutations

By dividing the sample into thousands of partitions, rare genetic variants can be detected with high sensitivity, even if they are only present in a very small proportion of the DNA.

Copy Number Variation (CNV) Analysis

DPCR allows precise determination of gene copy numbers and is therefore ideal for analyzing gene amplifications or deletions in genetic studies and oncology.

Liquid biopsy analyses

The high sensitivity of the method enables the detection of circulating DNA, e.g. tumor-associated mutations in blood samples, and thus supports minimally invasive diagnostic and monitoring approaches.

Pathogen detection

Viral or bacterial nucleic acids can be detected very sensitively and quantitatively – even with low target concentrations or complex sample matrices.

Gene expression analyses

After a reverse transcription reaction, RNA can be quantified precisely and absolutely, which is particularly advantageous for low expression or small sample quantities.

Validation of sequencing data

Results from next-generation sequencing (NGS) can be reliably confirmed and quantitatively verified with dPCR.

Food and environmental analysis

DPCR is suitable for the quantitative determination of genetic markers, for example for the detection of GMOs or specific microorganisms in complex samples.

Features .

Full compatibility .

The Plexus digital-PCR Master Mixes for Roche® digital Lightcycler® are compatible with assays and dPCR reactions on the digital Lightcycler® devices.

The integrated dye allows you to use all the quality and performance parameters of the digital Lightcycler® software as usual.