Stool samples for microbiome analyses .
The OMNIgene®-GUT Kits OM-200 and OMR-205 meet all requirements for best sample quality and a realistic microbiome profile: The sample collection itself is simple and clean, perfect for self-sampling by patients or study participants. The stool sample is immediately homogenized in the tube, and the preservation solution stabilizes nucleic acids very effectively. This genetic snapshot of the microbiota is preserved for a long time even without refrigeration during storage and transport.
Hard Facts .
- Intuitive and user-friendly sample collection
- Minimization of microbial growth
- Minimization of DNA degradation
- Stabilization of DNA during temperature fluctuations (-20°C to 50°C)
- Very good signal-to-noise ratio in subsequent data analyses
Applications .
Perfect sample homogenization and stabilization is necessary to minimize biases of the gut microbiome. A wide variety of microbes can be reliably detected from the collected samples via downstream applications.
Macklaim, J., Tayeb, M., Dillane, C., Fragel, C., & Cunningham, L. (2021). The need for stabilization: short-term variation in microbiome profiles. DNA Genotek https://www.dnagenotek.com/ROW/pdf/PD-WP-00070.pdf
Doukhanine, E., Bouevitch, A., Pozza, L., & Merino, C. (2014). OMNIgene®-GUT enables reliable collection of high quality fecal samples for gut microbiome studies. DNA Genotek https://www.dnagenotek.com/ROW/pdf/PD-WP-00040.pdf
Doukhanine, E., Bouevitch, A., Brown, A., Gage LaVecchia, J., Merino, C., & Pozza, L. (2016). OMNIgene®-GUT stabilizes the microbiome profile at ambient temperature for 60 days and during transport. DNA Genotek https://www.dnagenotek.com/ROW/pdf/PD-WP-00042.pdf
Brown, A., Lynch, D., Bouevitch, A., & Doukhanine, E. (2018). OMNIgene®-GUT provides easy self-collection and stabilization of liquid fecal samples for microbiome profiling. DNA Genotek. https://www.dnagenotek.com/ROW/pdf/PD-WP-00056.pdf
Tayeb, M., Macklaim, J., Colemeco, S., Fragel, C., Dillane, C., & Cunningham, L. (2020). Impact of fecal collection methods on sample homogenization and extraction reproducibility in gut microbiome profiling. DNA Genotek https://www.dnagenotek.com/ROW/pdf/PD-WP-00064.pdf
16S rRNA analyses enable the detection, identification and characterization of the microorganisms of the gut microbiome.
Anderson, E. L., Li, W., Klitgord, N., Highlander, S. K., Dayrit, M., Seguritan, V., Yooseph, S., Biggs, W., Venter, J. C., Nelson, K. E., & Jones, M. B. (2016). A robust ambient temperature collection and stabilization strategy: Enabling worldwide functional studies of the human microbiome. Scientific Reports, 6(1), 31731. https://doi.org/10.1038/srep31731
Choo, J. M., Leong, L. E., & Rogers, G. B. (2015). Sample storage conditions significantly influence faecal microbiome profiles. Scientific Reports, 5(1), 16350. https://doi.org/10.1038/srep16350
The human gut microbiome is increasingly becoming the focus of research. There is increasing evidence that the microbiome plays a crucial role in many physiological, pharmacokinetic and pathological processes.
Abrahamson, M., Hooker, E., Ajami, N. J., Petrosino, J. F., & Orwoll, E. S. (2017). Successful collection of stool samples for microbiome analyses from a large community-based population of elderly men. Contemporary Clinical Trials Communications, 7, 158-162. https://doi.org/10.1016/j.conctc.2017.07.002
OMNIgene GUT - OM-200 .
Product overview .
| Attribute | OM-200 | OMR-205 |
|---|---|---|
| Collection site | Feces | Feces |
| Sample homogenization | ✔ | ✔ |
| Microbial nucleic acids | DNA | DNA and RNA |
| Sample collected per kit (median) | 520 ± 101 mg | 545 mg |
| Shipping at ambient temperature | ✔ | ✔ |
| Compatible with a wide variety of nucleic acid extractions and purification methods | ✔ | ✔ |
| Standardized format for high-throughput processing | ✔ | ✔ |
| Number of extractions per kit (250 µL per extraction) | 8 | 10 (200 µl per extraction) |
| DNA yield per extraction ⌀ (median) | 12 ± 1.49 µg (12.56 µg) | 4.40 ± 0.52 µg (2.06 µg) |
| High molecular weight DNA | ✔ | ✔ |
| Microbiome DNA stability at room temperature | 60 days | 30 days |
| Microbiome RNA stability at room temperature | – | 10 days |
| Suitable for NGS downstream application | ✔ | ✔ |
| Suitable for long-term storage and biobanking | ✔ | ✔ |
Downloads .
PD-WP-00070: The need for stabilization: Short-term variation in microbiome profiles 0.00 KB
PD-WP-00064: Impact of stool collection methods on sample heterogeneity and extraction reproducibility in gut microbiome profiling 0.00 KB
PD-WP-00056: OMNIgene®•GUT provides easy self-collection and stabilization of liquid fecal samples for microbiome profiling. 0.00 KB
PD-WP-00042: OMNIgene®•GUT stabilizes the microbiome profile at ambient temperature for 60 days and during transport 0.00 KB
PD-WP-00040: OMNIgene®•GUT enables reliable collection of high quality fecal samples for gut microbiome studies 0.00 KB
OMNIgene®•GUT is compatible with the Shoreline complete microbiome prep kit for NGS 0.00 KB