Saliva kits for the recovery of DNA and RNA .

DNA Genotek‘s kits provide DNA and RNA in excellent quality and high yield. Other highlights include easy sample collection, safe handling and perfect stabilization of nucleic acids over weeks at room temperature. This makes transporting samples to the laboratory or study center safe and easy. DNA Genotek’s kits have also proven excellent for self-collection of samples by participants of studies.

Hard Facts .

Proven millions of times worldwide
Non-invasive method, safe handling
Long shelf life, even without cooling
High DNA yield, excellent DNA quality
Excellent performance in subsequent assays
Best suited for sample collection at home

Applications .

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Accordion Content

Genotyping

By detecting minute genetic changes – single nucleotide polymorphisms (SNPs) – potential disease risks or pathological changes can be identified.

Reiner, J., Karger, L., Cohen, N., Mehta, L., Edelmann, L., & Scott, S. A. (2017). Chromosomal Microarray Detection of Constitutional Copy Number Variation Using Saliva DNA. The Journal of Molecular Diagnostics, 19(3), 397-403. https://doi.org/10.1016/j.jmoldx.2016.11.006

Kushner, S. C., Herzhoff, K., Vrshek-Schallhorn, S., & Tackett, J. L. (2018). Depression in early adolescence: Contributions from relational aggression and variation in the oxytocin receptor gene. Aggressive Behavior, 44(1), 60-68. https://doi.org/10.1002/ab.21724

Epigenetic studies

Human DNA obtained with Oragene saliva kits is ideal for studying epigenetic changes.

Rushing, A., Sommer, E. C., Zhao, S., Po’e, E. K., & Barkin, S. L. (2020). Salivary epigenetic biomarkers as predictors of emerging childhood obesity. BMC Medical Genetics, 21(1), 34. https://doi.org/10.1186/s12881-020-0968-7

Telomere length determination

The excellent stabilization of DNA in the ORAgene Saliva Kits offers best conditions for telomere length determination.

Goldman, E. A., Eick, G. N., Compton, D., Kowal, P., Snodgrass, J. J., Eisenberg, D. T. A., & Sterner, K. N. (2018). Evaluating minimally invasive sample collection methods for telomere length measurement. American Journal of Human Biology, 30(1), e23062. https://doi.org/10.1002/ajhb.23062

HLA typing

Traditionally, bone marrow donor registries and transplant centers collect DNA samples from potential donors through either a blood sample or buccal swab. In contrast, sample collection with ORAgene kits is non-invasive and still provides high quality DNA for HLA typing.

DNA Genotek. (n.d.). HLA typing using DNA from oral samples collected with ORAcollect®-DNA. https://www. dnagenotek.com/ROW/pdf/PD-WP-00026.pdf

Epidemiological studies

Epidemiological studies – e.g. on the incidence of type 2 diabetes mellitus in the population – require easy sample collection in order to achieve a high willingness to donate on the part of the subjects.

James, G., McMullin, M. F., Duncombe, A. S., Clarke, M., & Anderson, L. A. (2018). The MOSAICC study: Assessing feasibility for biological sample collection in epidemiology studies and comparison of DNA yields from saliva and whole blood samples. Annals of Human Genetics, 82(2), 114-118. https://doi.org/10.1111/ahg.12227

McElfish, P. A., Narcisse, M.-R., Long, C. R., Ayers, B. L., Hawley, N. L., Aitaoto, N., Riklon, S., Su, L. J., Ima, S. Z., Wilmoth, R. O., Schulz, T. K., & Kadlubar, S. (2017). Leveraging community-based participatory research capacity to recruit Pacific Islanders into a genetics study. Journal of Community Genetics, 8(4), 283-291. https://doi.org/10.1007/s12687-017-0313-9

Personalized medicine

Genetic analyses help to individualize a patient’s therapy.

Details .

Various kits for sampling from saliva .

Item number	Saliva volume	Swab	DNA/RNA	⌀ Yield	Pediatric use	Sample stability at RT
OG-600 [OG-500]	2 ml	No	DNA	58.5 µg	No	> 5 years [2]
OG-610 [OG-510]	1 ml	No	DNA	36.0 µg	No	> 5 years [2]
ON-600 [ON-500]	0.5 ml	No	DNA	11.6 µg	No	Months
OG-675 [OG-575]	0.75 ml	Yes	DNA	17.3 µg	Yes	> 5 years [2]
OMR-610	1 ml	No	DNA and RNA	7.2 µg (DNA) 5.6 µg (RNA)	No	21 days
OC-175	–	Yes	DNA	2 µg	Yes	> 1 year
OCR-100	–	Yes	DNA	2.9 µg	Yes	Months
ORE-100	–	Yes	RNA	5.9µg	Yes	< 60 days

[1] Iwasiow, R.M., Tayeb, M.A. and James, C.M.P. (2009). Quality, yield and bacterial content of DNA from human saliva collected and purified using Oragene•ONE. DNA Genotek. PD-WP-013.
[2] Iwasiow, R.M.; Desbois, A.; Birnboim, H.C. (2011). Long-term stability of DNA from saliva samples stored in the Oragene® self-collection kit. DNA Genotek. PD-WP-005.
[3] Panford-Walsh, R.; Doukhanine, E.; Bouevitch, A. (2012). Comparison of DNA from samples collected using ORAcollect®•DNA (OCR‑100) vs. buccal swabs. DNA Genotek. PD-WP-0021.
[4] Niles, J.O., Rabuka, S., and Iwasiow, R.M. (2010). Non-invasive, assisted collection of high quantity and quality genomic DNA from saliva of young children. DNA Genotek. PD-WP-018.

Perfect stabilization of DNA in Oragene® kits .

The DNA in the saliva samples collected with Oragene® kits OG-600, OG-610, or OG-675 is stable at room temperature for several years. Expensive cooling or freezing of the samples during transport and storage can therefore be dispensed with.

From: Iwasiow, R. M., Desbois, A., & Birnboim, H. C. (2011). Long-term stability of DNA from saliva samples stored in the Oragene® self-collection kit. DNA Genotek. https://www.dnagenotek.com/ROW/pdf/PD-WP-005.pdf

DNA from Oragene saliva samples after 5 years of storage at room temperature.

OG-600 .

The OG-600 kit is one of our best sellers. This product provides an optimized all-in-one system for the collection, stabilization and transport of saliva samples for DNA recovery.

Advantages .

High quality and quantity of DNA
Equivalent to blood for DNA analysis
Painless and reliable self or assisted sampling
DNA is stable at room temperature for years
Optimized for both manual sample processing and automated workflows
Integrated 1D barcode + optional 2D floor barcode for efficient workflow
Proven in targeted sequencing and whole genome sequencing.

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

White Paper
White Paper

OG-610 .

Every genetic analysis begins with the collection of DNA samples. The OG-610 kit provides an optimized all-in-one system for the collection, stabilization and transport of saliva samples for DNA collection.

Advantages .

Painless and reliable self or assisted sampling
Equivalent to blood for DNA analysis
DNA is stable at room temperature for years
Optimized for both manual sample processing and automated workflows
Integrated 1D barcode + optional 2D floor barcode for efficient workflow
Proven in targeted sequencing and whole genome sequencing.

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

White Paper
White Paper

OG-675 .

This product has been developed by DNA Genotek especially for the small patients. With the help of an absorbent sponge, DNA can be extracted from saliva in large quantities and high quality. The DNA is also perfectly stabilized in this product.

Advantages .

High quality and quantity of DNA
Painless and reliable sampling assisted by an adult
Equivalent to blood for DNA analysis
DNA is stable at room temperature for years
Optimized for both manual sample processing and automated workflows
Integrated 1D barcode + optional 2D floor barcode for efficient workflow
Proven in targeted sequencing and whole genome sequencing.

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

White Paper
White Paper

OMR-610 .

The OMNIgene-SALIVA DNA and RNA Kit is validated for self-collection of saliva and total nucleic acid stabilization. Single sample analysis with a true multi-omics collection kit sets a new standard for home collection of total nucleic acids from saliva.

Advantages .

Collection of human and microbial DNA and RNA in one kit
DNA and RNA is stable at room temperature for up to 21 days
Equivalent to blood for DNA analysis
Painless and reliable self-sampling
Optimized for both manual sample processing and automated workflows
Integrated 1D barcode for efficient workflow
Compatible with downstream applications, including PCR/RT-PCR

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

ON-600 .

Every genetic analysis begins with the collection of DNA samples. Collect reliable samples with an all-in-one system for the collection, stabilization and transport of DNA from saliva.

Advantages .

Painless and reliable self or assisted sampling
Equivalent to blood for DNA analysis
DNA is stable at room temperature for years
Optimized for both manual sample processing and automated workflows
Integrated 1D barcode + optional 2D floor barcode for efficient workflow
Proven in targeted sequencing and whole genome sequencing.

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

OCR-100 .

ORAcollect-DNA is a painless, non-invasive and easy-to-use DNA collection kit for self or assisted sample collection. ORAcollect DNA is designed to collect, stabilize and protect DNA samples. DNA from ORAcollect DNA can be used for genetic testing. Unlike buccal swabs, collected specimens are liquid, bacteriostatic, and easily integrated into existing laboratory workflows.

Advantages .

High-quality DNA for use in downstream molecular applications.
Painless and reliable self or assisted sampling
Sample does not require drying time
Bacteriostatic - inhibits the growth of bacteria from sampling to processing
Complete traceability of samples through integrated barcodes
Easy processing due to compatibility with robotics and automation

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

White Paper
White Paper

ORE-100 .

Every expression analysis starts with the collection of RNA samples. ORAcollect® RNA (ORE-100) is an all-in-one system for the collection, stabilization and transport of RNA from saliva for in vitro diagnostics.

Advantages .

Stabilizes samples during collection and ensures that DNA and RNA are unchanged at the time of processing
Stabilizes human, viral and bacterial RNA from saliva at room temperature
Reduces transportation and storage costs and complexity with up to 60 days stability of RNA at room temperature
Easy self-collection of high-quality DNA and RNA from oral samples
No refrigerated transports necessary
Compatible with downstream applications (e.g. RT-qPCR, microarray)
Format suitable for high throughput processing

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

OC-175 .

ORAcollect for pediatrics is a painless and easy-to-use DNA collection kit that can be used by parents or trained professionals. Designed for young donors, the kit provides a non-invasive DNA collection that is gentle and quick for both the donor and the assisting person. After collection, the sample is stabilized so that immediate extraction and analysis in the laboratory is not required.

Advantages .

Painless sample collection - in contrast to heel pricks or blood sampling
Fast sampling protocol without waiting times
High-quality DNA for all molecular downstream applications
Bacteriostatic - inhibits the growth of bacteria from sampling to processing
Protected sample - DNA remains stable at room temperature for 1 year
Complete traceability of samples through integrated barcodes
Easy processing due to compatibility with robotics and automation

More information .

Manufacturer website
Manufacturer website

Instruction manual (engl.)
Instruction manual (engl.)

Data Sheet
Data Sheet

ORAgene Saliva Kits .

DNA kits for research – versatile in use.

Downloads .

Data Sheets

Manuals

Safety Data Sheets

White Paper

Storage and transport

Protocols

Data Sheets

Manuals

Safety Data Sheets

White Paper

Storage and transport

Protocols

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Saliva kits for the recovery of DNA and RNA .

Hard Facts .

Applications .

Details .

Various kits for sampling from saliva .

ORAgene Saliva Kits .

Human DNA Collection Kit; Pädiatrie – OC-175

Saliva DNA Collection Kit – OCR-100

Saliva Self Collection Kit; 2 ml – OG-600

Saliva Self Collection Kit; 1 ml – OG-610

Saliva Self Collection Kit; 0.75 ml – OG-675

Saliva Self Collection Kit; 0.5 ml – ON-600

RNA Self Collection Kit – ORE-100

Downloads .

DNA Genotek – Human Genomic Kits – Overview 1.02 MB

OMNIGene SALIVA DNA and RNA – OMR-610 – Fact Sheet 1.29 MB

ORAcollect DNA – OCR-100 – Fact Sheet 0.00 KB

ORAcollect – OC-175 – Fact Sheet 0.00 KB

ORAcollect RNA – ORE-100 – Fact Sheet 0.00 KB

ORAgene DNA – OG-600 – Fact Sheet 0.00 KB

ORAgene DNA – 600 series – Weight and Dimension 0.00 KB

ORAgene DNA – OG-610 – Fact Sheet 0.00 KB

ORAgene DNA – OG-675 – Fact Sheet 0.00 KB

ORAgene ONE – ON-600 – Fact Sheet 0.00 KB

OMNIGene SALIVA DNA and RNA – OMR-610 – Manual 0.00 KB

ORAcollect DNA – OCR-100 – Manual 0.00 KB

ORAcollect – OC-175 – Manual 0.00 KB

ORAcollect RNA – ORE-100 – Manual 0.00 KB

ORAgene DNA – OG-600/OG-610 – Manual 0.00 KB

ORAgene DNA – OG-675 – Manual 0.00 KB

ORAgene ONE – ON-600 – Manual 0.00 KB

OMNIGene SALIVA DNA and RNA – OMR-610 – Safety Data Sheet 0.00 KB

ORAcollect DNA – OCR-100 – Safety Data Sheet 0.00 KB

ORAcollect – OC-175 – Safety Data Sheet 0.00 KB

ORAcollect RNA – ORE-100 – Safety Data Sheet 0.00 KB

ORAgene DNA – OG-600/OG-610/OG-675 – Safety Data Sheet 0.00 KB

ORAgene ONE – ON-600 – Safety Data Sheet 0.00 KB

PD-WP-00052 – Can saliva replace blood for DNA collection and analysis? 0.00 KB

PD-WP-001: Expected DNA yield from Oragene/saliva samples 0.00 KB

PD-WP-00031: Impact of population and laboratory methods on DNA yield and variability 0.00 KB

PD-WP-011: Human genomic vs bacterial DNA content of Oragene/saliva samples 0.00 KB

PD-WP-012: Comparison of DNA yield from Oragene/saliva and blood samples 0.00 KB

PD-WP-005: Long-term stability of DNA from saliva samples stored in the Oragene® self-collection kit 0.00 KB

PD-PR-012: Long-term storage of Oragene®/saliva samples 0.00 KB

0.5 mL (500 μL) aliquot using DNA Genotek’s prepIT•L2P 0.00 KB

4 ml whole sample using DNA Genotek’s prepIT•L2P 0.00 KB