DNA/RNA extraction from pathogens .
The MagSi-NA and MagSi-DX Pathogens kit provide nucleic acids with high purity and yield for qPCR-based or other enzymatic pathogen detection methods.
The MagSi-NA and MagSi-DX Pathogens Kits can be used to obtain DNA and RNA from a wide variety of sample materials, including serum, plasma, oropharyngeal swabs, nasopharyngeal swabs, and respiratory samples.
Hard Facts .
- High yield of DNA/RNA
- Time required for 96 samples <30 min
- No cooling, centrifugation or filtration required
- Compatible with Kingfisher™ and other automation solutions
- Optimized for lysates with high viscosity
- Suitable for many downstream applications
- CE-IVD certified (MagSi-DX Pathogen)
Applications .
For manual and automated purification of RNA for the diagnosis of human samples (respiratory swabs, saliva), the CE-IVD certified MagSi-DX Pathogen Kit is recommended. The purified RNA is suitable for all common methods for the detection of viral SARS-CoV-2 RNA, especially RT-qPCR and sequencing.
Jonckheere, I., Faes, L., Overmeire, Y., De Vleeschauwer, A., Vanden Daele, L., Van Bruaene, N., Vandecandelaere, I., Merlaen, B., van Cann, J., & Vandesompele, J. (2021). Equivalence of saliva RT-qPCR testing to nasal-throat/nasopharyngeal swab testing in the general practitioner’s setting to detect SARS-CoV-2 [Preprint]. Infectious Diseases (except HIV/AIDS). https://doi.org/10.1101/2021.09.30.21264181
Manual and automated purification of RNA for virus detection by PCR, qPCR and LAMP.
The MagSi-NA or CE-IVD certified MagSi-DX Pathogen Kit allow low-cost extraction of DNA and RNA from a variety of sample materials: serum, plasma, oropharyngeal and nasopharyngeal swabs or other respiratory samples. The isolated nucleic acids can be used for qPCR or other common pathogen detection methods.
Magtivio. (n.d.). MagSi-NA Pathogens – Fast and cost-effective extraction of total nucleic acids for pathogen detection. https://www.magtivio.com/wp-content/uploads/2021/12/PL0054-002_MagSi-NA_Pathogens.pdf
MagSi-DX Pathogen / MagSi-NA Pathogen .
Simple workflow, fast and cost-effective .
The purification of RNA and DNA using MagSi beads is very simple and fast.
The protocol consists of only four steps: lysis, NA binding, washing, eluting.
The lysed sample material is mixed with the Magnetic Beads. The nucleic acids bind to the beads, which can be easily separated and washed using a magnet. In the final step, the bound DNA is released from the beads again using the elution buffer.
The MagSi-NA and the MagSi-DX Pathogen Kit can be used with Kingfisher™, PurePrep™, Hamilton® and TECAN® systems, among others.
Kits for DNA/RNA extraction from pathogens .
All kits are available in different package sizes.
