Collection Kits for Intestinal Metabolome Analysis .
Metabolome refers to the totality of metabolites in a functional unit or organ, such as the intestine.
The metabolites in stool samples basically represent the biochemical processes occurring in the intestine. It is quite likely that the signatures from metabolome profiles are closely related to human health, well-being and also pathological processes.
OMNImet®-GUT (ME-200) is an all-in-one system for easy collection, homogenization and stabilization of faecal samples at room temperature.
Key Facts .
- Easy self-sampling at home
- Metabolome profile is stable at room temperature for 7 days
- Optimized for metabolome-wide analyses
- Only one sample needed for all metabolomic assays
- Very high reproducibility due to point-of-collection sample homogenization
- Easy to automate, suitable for high throughput processing
- Compatible with all popular analysis platforms
Applications .
Short-chain fatty acids (SCFAs) are key mediators of microbial activity in the gut. They play an important role in regulating the immune system, maintaining the epithelial barrier of the gut, and preventing diseases such as metabolic syndrome, inflammatory bowel disease, and certain types of cancer.
De Bruin, O. M., Freinkman, E., Breton, H., Evans, A. M., Miller, L. A. D., Nguyen, U., Adam, K.-P., & Doukhanine, E. (2020). OMNImetTM-GUTenables at-home collection and ambient temperature transport of fecal samples for metabolomics. DNA Genotek https://www.dnagenotek.com/ROW/pdf/PD-WP-00066.pdf
Metabolites play an important role in the communication and dynamics between the gut microbiome and the host. Metabolomics is therefore a particularly powerful tool for analyzing and elucidating the functions of the gut microbiome in diseases such as obesity, insulin resistance, heart disease, and Parkinson’s disease.
The OMNImet™-GUT ME-200 kit provides a solution to stabilize metabolome profiles without interfering with subsequent analyses on LC/MS platforms.
De Bruin, O. M., Freinkman, E., Breton, H., Evans, A. M., Miller, L. A. D., Nguyen, U., Adam, K.-P., & Doukhanine, E. (2020). OMNImetTM-GUTenables at-home collection and ambient temperature transport of fecal samples for metabolomics. DNA Genotek https://www.dnagenotek.com/ROW/pdf/PD-WP-00066.pdf
OMNImet GUT ME-200 .
Product overview .
| Attribute | OMNImet GUT (ME-200) |
|---|---|
| Fecal sample collected per kit (median) [1] | 520 ± 101 mg |
| Metabolomic profile stability at room temperature [2] | 7 days |
| Validated for untargeted discovery metabolomics [2] | ✔ |
| Validated for both targeted quantification of short-chain fatty acids (SCFAs) [2] | ✔ |
| Preserve metabolites integrity through one freeze-thaw cycle [2] | -20ºC to +30ºC |
| Compatible with common analytical platforms | ✔ |
| Suitable for long-term storage and biobanking | ✔ |
| Bar coded for full sample traceability (128C) | ✔ |
[2] DNA Genotek, validation done by Metabolon® (OMNImet™•GUT enables at-home collection and ambient temperature transport of fecal samples for metabolomics. PD-WP-00066)
