Quantitative PCR (qPCR) - Steinbrenner Laborsysteme GmbH

Quantitative PCR

Only the best for your qPCR.

Our primaQUANT Master Mixes for SYBR® Green- or probe-based qPCR guarantee reliable and reproducible results. They are characterized by high sensitivity, great robustness and excellent sensitivity. The detection limit is below 10 pg DNA.
The primaQUANT qPCR Master Mixes, like all our molecular biology reagents, are developed and produced entirely in Germany.

Many possibilities .

Genotyping

RNA / miRNA expression

Diagnostics

Quality control

1-Step and 2-Step

probes or SYBR® Green

HotStart

Ready-to-use

Features .

Excellent performance .

The primaQUANT ADVANCED master mixes offer a wide dynamic range and excellent performance, even with low template amounts or targets with low expression.

For all qPCR cyclers .

Our qPCR 2x Master Mixes are suitable for all common qPCR cyclers and optionally available with ROX.

We have compiled a table for you showing whether your device uses ROX(6-carboxy- X-rhodamine) for normalization and which concentration, if any, is required.

qPCR in less than 20 minutes .

Extremely fast

The primaQUANT qPCR Master Mixes cope with the shortest cycle times due to their ultra fast DNA polymerase: a complete qPCR run takes less than 20 minutes under suitable conditions.

Of course, you can also use your previous qPCR protocol.

Standard protocol:

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	Step	Time	Temperature
	Initial denaturation	1-3 minutes	92°C – 95°C
25 – 40 Cycles	Denaturation	5-10 seconds	92°C – 95°C
	Combined Annealing/Elongation	10-20 seconds	60°C – depending on used primers

Fast protocol:

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	Step	Time	Temperature
	Initial denaturation	1 minute	92°C – 95°C
25 – 40 Zyklen	Denaturation	5 seconds	92°C – 95°C
	Combined annealing/elongation	10 seconds	60°C – depending on used primers

Ultra Fast Protocol:

Save as
xlsx
pdf
print

	Step	Time	Temperature
	Initial denaturation	1 minute	92°C – 95°C
25 – 40 cycles	Denaturation	1 second	92°C – 95°C
	Combined Annealing/Elongation	1-5 seconds	60°C – depending on used primers

Temperature-stable .

Freeze/thaw

Our PCR and qPCR reagents are not affected by multiple freezing/thawing . You can therefore freeze and thaw the mixes more than 10 times without any problems.

Storage in the refrigerator

The primaQUANT Master Mixes are stable for weeks at 4°C. For short-term storage, we generally recommend storage at 4°C in the refrigerator.

Stability at room temperature

The primaQUANT Master Mixes can even be stored for several days at room temperature without loosing quality. Therefore, you no longer have to pipette your qPCR laboriously on ice to avoid a loss of sensitivity!

Smart Package Size .

No more annoying aliquoting!

Our Master Mixes come in units of 1 ml as standard.

This is very convenient and practical, because one tube is enough for exactly one 96 well plate, two tubes for one 384 well plate. If you ever need less, you can simply store the mixes in the refrigerator for several weeks.

Customized fillings: As the manufacturer, we can of course supply you with fillings and mixtures according to your special requirements.

Made in Germany .

We develop and produce our enzymes and master mixes exclusively ourselves in Germany. You benefit from this in several ways:

Our qPCR product groups .

For most applications we can offer the right product – just ask us.

qPCR (SYBR® Green)

Advanced Master Mixes with SYBR® Green for qPCR

qPCR (sample)

Advanced Master Mixes for probe-based qPCR, e.g. Taqman®.

qPCR (SYBR® Green)

Master Mixes with SYBR® Green for qPCR

qPCR (sample)

Master Mixes for probe-based qPCR, e.g. Taqman®.

1-Step RT-qPCR for probes

Master mixes for one-step RT-qPCR without separate rewrite of mRNA.

1-Step RT-qPCR with SYBR® Green

Master mixes for one-step RT-qPCR without separate rewrite of mRNA.

Would you like more information or a demo?

Then please feel free to contact us.
We look forward to your call at +49 (0) 6223 96 73 00 or an email.

You want to transcribe RNA into cDNA ?

For the determination of RNA expression, detection of RNA viruses or RNA-seq, the (m)RNA to be detected must first be transcribed into cDNA.
With our primaREVERSE RT kit, you synthesize cDNA with high efficiency and in the shortest possible time for your downstream applications.

Extremely fast: cDNA in under 15 minutes
High yield due to reduced RNase-H activity
Ready-to-use: complete kit incl. Primer and DNase
For templates up to 17 kb in length
Also for complex transcripts

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Steinbrenner Laborsysteme GmbH

Quantitative PCR

Only the best for your qPCR.

Many possibilities .

Genotyping

RNA / miRNA expression

Diagnostics

Quality control

1-Step and 2-Step

probes or SYBR® Green

HotStart

Ready-to-use

Features .

Excellent performance .

Our qPCR product groups .

qPCR (SYBR® Green)

qPCR (sample)

qPCR (SYBR® Green)

qPCR (sample)

1-Step RT-qPCR for probes

1-Step RT-qPCR with SYBR® Green

Would you like more information or a demo?

You want to transcribe RNA into cDNA ?