Quantitative PCR (qPCR) - Steinbrenner Laborsysteme GmbH

Quantitative PCR

Only the best for your qPCR.

Our primaQUANT Master Mixes for SYBR® Green- or probe-based qPCR guarantee reliable and reproducible results. They are characterized by high sensitivity, great robustness and excellent sensitivity. The detection limit is below 10 pg DNA.
The primaQUANT qPCR Master Mixes, like all our molecular biology reagents, are developed and produced entirely in Germany.

Many possibilities .

Genotyping

RNA / miRNA expression

Diagnostics

Quality control

1-Step and 2-Step

probes or SYBR® Green

HotStart

Ready-to-use

Features .

For all qPCR cyclers .

Our qPCR 2x Master Mixes are suitable for all common qPCR cyclers and optionally available with ROX.

We have compiled a table for you showing whether your device uses ROX(6-carboxy- X-rhodamine) for normalization and which concentration, if any, is required.

qPCR in less than 20 minutes .

Extremely fast

The primaQUANT qPCR Master Mixes cope with the shortest cycle times due to their ultra fast DNA polymerase: a complete qPCR run takes less than 20 minutes under suitable conditions.

Of course, you can also use your previous qPCR protocol.

Standard protocol:

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	Step	Time	Temperature
	Initial denaturation	1-3 minutes	92°C – 95°C
25 – 40 Cycles	Denaturation	5-10 seconds	92°C – 95°C
	Combined Annealing/Elongation	10-20 seconds	60°C – depending on used primers

Fast protocol:

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	Step	Time	Temperature
	Initial denaturation	1 minute	92°C – 95°C
25 – 40 Zyklen	Denaturation	5 seconds	92°C – 95°C
	Combined annealing/elongation	10 seconds	60°C – depending on used primers

Ultra Fast Protocol:

Save as
xlsx
pdf
print

	Step	Time	Temperature
	Initial denaturation	1 minute	92°C – 95°C
25 – 40 cycles	Denaturation	1 second	92°C – 95°C
	Combined Annealing/Elongation	1-5 seconds	60°C – depending on used primers

Highly Specific .

Our primaQUANT PROBE qPCR Master Mixes work very reliably under a wide range of assay conditions.

They are very robust with respect to primer concentration and require extremely little sample material.

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Excellent performance .

Convincing values for all important performance parameters guarantee reliable and reproducible results even under difficult conditions.

Always stable .

Freeze/thaw

Our PCR and qPCR reagents are not affected by multiple freezing/thawing . You can therefore freeze and thaw the mixes more than 10 times without any problems.

Refrigerator storage

The primaQUANT Master Mixes are stable for weeks at 4°C. For short-term storage, we generally recommend storage at 4°C in the refrigerator.

Stability at room temperature

The primaQUANT Master Mixes can even be stored for several days at room temperature without loosing quality. Therefore, you no longer have to pipette your qPCR laboriously on ice to avoid a loss of sensitivity!

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Smart Package Size .

No more annoying aliquoting!

Our Master Mixes come in units of 1 ml as standard.

This is very convenient and practical, because one tube is enough for exactly one 96 well plate, two tubes for one 384 well plate. If you ever need less, you can simply store the mixes in the refrigerator for several weeks.

Customized fillings: As the manufacturer, we can of course supply you with fillings and mixtures according to your special requirements.

Coloring for optimized handling .

Some of the primaQUANT Master Mixes are optionally available dyed blue. This makes pipetting much more convenient and also much safer. In this way, you can immediately see whether mastermix has already been added to a preparation and which wells have already been pipetted or possibly pipetted. have been forgotten.

The dye used has no adverse effect on qPCR.

Made in Germany .

We develop and produce our enzymes and master mixes exclusively ourselves in Germany. You benefit from this in several ways:

Our qPCR product groups .

For most applications we can offer the right product – just ask us.

qPCR (SYBR® Green)

Master Mixes with SYBR® Green for qPCR

qPCR (sample)

Master Mixes for probe-based qPCR, e.g. Taqman®.

1-Step RT-qPCR for probes

Master mixes for one-step RT-qPCR without separate rewrite of mRNA.

1-Step RT-qPCR with SYBR® Green