Animal microbiome and genetics .
Our PERFORMAgene and PERFORMAbiome GUT kits are ideal for painless, non-invasive sample collection from animals. PERFORMAgene is an easy to use all-in-one system for oral, nasal or vaginal sample collection for genetic analysis. PERFORMAbiome GUT enables easy sample collection for analysis of microbiome profiles in animals.
Hard Facts .
- Painless, non-invasive sampling of animals
- No cold chain required
- High tolerance for ambient temperature fluctuations
- All-in-one system for collection, stabilization and transport
- High yield of high quality DNA
Applications .
Animal stool samples can be collected using the PERFORMAbiome-GUT PB-200 kit for diagnostics or analysis of the gut microbiome. The kit stabilizes the microbiome at the time of collection and enables reliable analyses.
Lin, C.-Y., Cross, T.-W. L., Doukhanine, E., & Swanson, K. S. (2020). An ambient temperature collection and stabilization strategy for canine microbiota studies. Scientific Reports, 10(1), 13383. https://doi.org/10.1038/s41598-020-70232-6
Traditionally, genetic analyses of animals were performed using blood or tail hair samples. PERFORMAgene PG-100 kits enable sampling with nasal, oral and vaginal swabs – increased compliance with consistent high quality DNA for subsequent analyses.
DNA Genotek. (n.d.). Case Study: Nasal samples as an alternative source of high yield, high quality genomic DNA for livestock genotyping. https://www.dnagenotek.com/US/pdf/MK-00078.pdf
For producers in the dairy industry, genetic testing of their livestock and young animals is essential. The standard method is DNA collection from blood, tail hair or ear punch samples. These come with many drawbacks – including more difficult transportation options, DNA yields that don’t scale, and the need for trained workers. With the Swab-based PG-100 system, DNA samples can be easily collected and stabilized through a nasal, oral or vaginal swab.
DNA Genotek. (n.d.). Case Study: Holstein UK uses PERFORMAgene to collect 400 samples in parentage analysis of herdbook. https://www.dnagenotek.com/US/pdf/MK-00231.pdf
Details .
High quality DNA samples for animal genetics .
PERFORMAgene (PG-100) is a simple, non-invasive, all-in-one kit for the collection, stabilization and transport of DNA in animal swab samples. Our saliva collection kits for animals are easy to use and have proven high reliability in all environments where DNA samples are collected – be it in the barn, in the field, in the clinic or at home.
Proven performance for a range of animal applications including genotyping, sequencing, parentage, DNA diagnostics and bio-banking.
Ideal for use in livestock (cattle, sheep, goats), companion animals (dogs, cats, horses) and other species by researchers, breeders, laboratories and consumers.
- Painless, non-invasive removal ensures optimal animal welfare
- High-quality and quantitative DNA that enables multiple assays from a single sample
- Proven performance in livestock and companion animals for parentage, breed identity, hereditary disease testing, genotyping, sequencing, and sufficient samples for biobanking.
- The DNA sample is stable for 1 year at room temperature and for 28 months before collection
- Validated for multiple PCR assays and on Illumina, Affymetrics and Fluidigm platforms.
- Reliable in all collection environments – barn, field, clinic or home.
- Samples can be sent by regular mail without a cold chain, making them ideal for mailing studies.
- Very low bacterial content, resulting in fewer false samples
- Barcode for complete traceability of the samples
- All-in-one system for collection, stabilization and transport
- Parentage, racial identity, genetic diseases, genetic traits.
- Breeding clubs, breed associations, producers/breeders, breeders
- Simple, non-invasive DNA sampling devices that allow for optimal animal welfare
- The DNA sample is stable for 1 year at room temperature; a cold chain is not required, so the sample can be sent via the normal postal system.
- Collection methods: nasal, oral or vaginal
- Reliable in all collection environments – barn, field, clinic or home.
- Collection at any age, from birth (newborns) to adult animals.
- Customizable packing
- All-in-one system for collection, stabilization and transport
- Painless, non-invasive removal ensures optimal animal welfare
- High-quality and quantitative DNA that enables multiple assays from a single sample
- Proven performance in livestock and companion animals for parentage, breed identity, hereditary disease testing, genotyping, sequencing, and sufficient samples for biobanking.
- The samples can be sent without cold chain by the normal postal system
- Barcoding for sample traceability throughout the entire workflow
- The DNA sample is stable for 1 year at room temperature after collection and for 28 months before collection
- The tube can be integrated into laboratory automation systems to increase efficiency
- Validated for multiple PCR assays and on Illumina, Affymetrics and Fluidigm platforms.
- Reliable in all collection environments – barn, field, clinic or home.
- Very low bacteria content leads to fewer false samples
- Customized packing possible
- All-in-one system for collection, stabilization and transport
Collect and stabilize DNA for quantitative analysis of the microbiome. .
PERFORMAbiome GUT (PB-200) is a simple all-in-one system for easy collection and stabilization of DNA from stool samples for analysis of gut microbiome profiles in animals.
Advantages .
- Minimization of distortions caused by microbial growth and DNA degradation.
- Elimination of costs associated with temperature-controlled shipping
- Eliminate the need for weighing and aliquoting for a standard volumetric sample.
- Maintenance of DNA integrity under typical ambient temperature fluctuations (-20°C to 50°C).
- Minimize noise in data analysis by providing a reliable microbiota profile
Attribute | PB-200 |
---|---|
Sample homogenization | ✔ |
Sample collected per kit (median) | 630 ± 120 mg |
Shipping at ambient temperature | ✔ |
Compatible with guanidinium based extraction kits | ✔ |
Standardized format for high-throughput processing | ✔ |
Number of extractions per kit (250 μL per extraction) | 8 |
DNA yield per extraction (median) | 1.66 ± 0.8 μg |
High molecular weight DNA | ✔ |
Microbiome profile stability at room temperature | 60 days |
Suitable for NGS downstream application | ✔ |
Quantitative analysis were performed using canine fecal samples | ✔ |