... Service for Science
... Service for Science
In der Au 17, 69257 Wiesenbach
+49 (0) 6223 / 96730-0
mail@steinbrenner.de
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| Name of PCR | |||
| Number of Reactions | 10 | ||
| Final Volume per Reaction | 20 | ul | |
| Reagents | Stock Concentration | Final Concentration | Volume to add [µl] |
| qPCR Master Mix [x-times concentrated] | 2 | 1 | 100 |
| Reverse Primer [µM] | 10 | 0.3 | 6 |
| Foward Primer [µM] | 10 | 0.3 | 6 |
| PCR-grade Water | 87 | ||
| Total Volume per reaction (without template) [µl] | 19.9 | ||
| Total Volume of Final Master Mix (without template) [µl] | 199 | ||
| cDNA/DNA Template | Volume within reaction [µl] | ||
| Stock Concentration | 100 | [ng/µl] | |
| Desired amount per well | 10 | ng per reaction | 0.1 |
| Aliquot | 1.9900 | µl per well of Final Master Mix | |
| add | 0.1 | µl per well of DNA/cDNA Template | |
