Reverse transcription and qPCR with SYBR® Green .
Hard Facts .
The primaQUANT 1 STEP RT-qPCR Master Mix allows reverse transcription and qPCR in one step. Thus, gene expression can be determined quickly via the RNA level without additional effort.
For the diagnosis of pathogenic RNA viruses, the pathogen RNA must be transcribed into cDNA. With the primaQUANT 1Step RT-qPCR Master Mix, you can complete reverse transcription and qPCR in one go. This makes high-throughput analysis and automation easy.
High Resultion Melting Curve Analysis (HRM) can be used to easily detect the smallest changes in the genome, such as SNP and mutations, using qPCR.
Matsuda, K. (2017). PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement. Advances in Clinical Chemistry, 80, 45-72. https://doi.org/10.1016/bs.acc.2016.11.002
Simko, I. (2016). High-Resolution DNA Melting Analysis in Plant Research. Trends in Plant Science, 21(6), 528-537. https://doi.org/10.1016/j.tplants.2016.01.004
Mohammad Rahimi, H., Pourhosseingholi, M. A., Yadegar, A., Mirjalali, H., & Zali, M. R. (2019). High-resolution melt curve analysis: A real-time based multipurpose approach for diagnosis and epidemiological investigations of parasitic infections. Comparative Immunology, Microbiology and Infectious Diseases, 67, 101364. https://doi.org/10.1016/j.cimid.2019.101364
For all qPCR cyclers .
Our qPCR 2x Master Mixes are suitable for all common qPCR cyclers and optionally available with ROX.
We have compiled a table for you showing whether your device uses ROX(6-carboxy- X-rhodamine) for normalization and which concentration, if any, is required.