Saliva kits for the recovery of DNA and RNA .
DNA Genotek‘s kits provide DNA and RNA in excellent quality and high yield. Further highlights are the easy sample collection, safe handling and perfect stabilization of the nucleic acids over days and weeks at room temperature. This makes transporting the samples to the laboratory or study center safe and easy. DNA Genotek’s kits have also proven to be excellent for taking samples from study participants at home.
Hard Facts .
- Proven millions of times worldwide
- Non-invasive method, safe handling
- Long shelf life, even without cooling
- High DNA yield, excellent DNA quality
- Excellent performance in subsequent assays
- Ideally suited for sampling at home
Applications .
By detecting minute genetic changes – single nucleotide polymorphisms (SNPs) – potential disease risks or pathological changes can be identified.
Reiner, J., Karger, L., Cohen, N., Mehta, L., Edelmann, L., & Scott, S. A. (2017). Chromosomal Microarray Detection of Constitutional Copy Number Variation Using Saliva DNA. The Journal of Molecular Diagnostics, 19(3), 397-403. https://doi.org/10.1016/j.jmoldx.2016.11.006
Kushner, S. C., Herzhoff, K., Vrshek-Schallhorn, S., & Tackett, J. L. (2018). Depression in early adolescence: Contributions from relational aggression and variation in the oxytocin receptor gene. Aggressive Behavior, 44(1), 60-68. https://doi.org/10.1002/ab.21724
Human DNA obtained with Oragene saliva kits is ideal for studying epigenetic changes.
Rushing, A., Sommer, E. C., Zhao, S., Po’e, E. K., & Barkin, S. L. (2020). Salivary epigenetic biomarkers as predictors of emerging childhood obesity. BMC Medical Genetics, 21(1), 34. https://doi.org/10.1186/s12881-020-0968-7
The excellent stabilization of DNA in the ORAgene Saliva Kits offers best conditions for telomere length determination.
Goldman, E. A., Eick, G. N., Compton, D., Kowal, P., Snodgrass, J. J., Eisenberg, D. T. A., & Sterner, K. N. (2018). Evaluating minimally invasive sample collection methods for telomere length measurement. American Journal of Human Biology, 30(1), e23062. https://doi.org/10.1002/ajhb.23062
Traditionally, bone marrow donor registries and transplant centers collect DNA samples from potential donors either through a blood sample or buccal swab. In contrast, sample collection with ORAgene kits is non-invasive and still provides high quality DNA for HLA typing.
DNA Genotek. (n.d.). HLA typing using DNA from oral samples collected with ORAcollect®-DNA. https://www. dnagenotek.com/ROW/pdf/PD-WP-00026.pdf
Epidemiological studies – e.g. on the occurrence of type 2 diabetes mellitus in the population – require simple sample collection in order to achieve a high willingness to donate on the part of the test subjects.
James, G., McMullin, M. F., Duncombe, A. S., Clarke, M., & Anderson, L. A. (2018). The MOSAICC study: Assessing feasibility for biological sample collection in epidemiology studies and comparison of DNA yields from saliva and whole blood samples. Annals of Human Genetics, 82(2), 114-118. https://doi.org/10.1111/ahg.12227
McElfish, P. A., Narcisse, M.-R., Long, C. R., Ayers, B. L., Hawley, N. L., Aitaoto, N., Riklon, S., Su, L. J., Ima, S. Z., Wilmoth, R. O., Schulz, T. K., & Kadlubar, S. (2017). Leveraging community-based participatory research capacity to recruit Pacific Islanders into a genetics study. Journal of Community Genetics, 8(4), 283-291. https://doi.org/10.1007/s12687-017-0313-9
Genetic analyses help to individualize a patient’s therapy.
Details .
Various kits for sampling from saliva .
| Item number | Saliva volume | Swab | DNA/RNA | ⌀ Yield | Pediatric use | Sample stability at RT |
|---|---|---|---|---|---|---|
| OG-600 [OG-500] | 2 ml | No | DNA | 58.5 µg | No | > 5 years [2] |
| OG-610 [OG-510] | 1 ml | No | DNA | 36.0 µg | No | > 5 years [2] |
| ON-600 [ON-500] | 0.5 ml | No | DNA | 11.6 µg | No | Months |
| OG-675 [OG-575] | 0.75 ml | Yes | DNA | 17.3 µg | Yes | > 5 years [2] |
| OMR-610 | 1 ml | No | DNA and RNA | 7.2 µg (DNA) 5.6 µg (RNA) | No | 21 days |
| OC-175 | – | Yes | DNA | 2 µg | Yes | > 1 year |
| OCR-100 | – | Yes | DNA | 2.9 µg | Yes | Months |
| ORE-100 | – | Yes | RNA | 5.9µg | Yes | < 60 days |
[2] Iwasiow, R.M.; Desbois, A.; Birnboim, H.C. (2011). Long-term stability of DNA from saliva samples stored in the Oragene® self-collection kit. DNA Genotek. PD-WP-005.
[3] Panford-Walsh, R.; Doukhanine, E.; Bouevitch, A. (2012). Comparison of DNA from samples collected using ORAcollect®•DNA (OCR‑100) vs. buccal swabs. DNA Genotek. PD-WP-0021.
[4] Niles, J.O., Rabuka, S., and Iwasiow, R.M. (2010). Non-invasive, assisted collection of high quantity and quality genomic DNA from saliva of young children. DNA Genotek. PD-WP-018.
ORAgene Saliva Kits .
Downloads .
White Paper
PD-WP-00031: Impact of population and laboratory methods on DNA yield and variability 0.00 KB