Functionalized beads .

The MagSi-Tools product line provides surface-activated magnetic beads. Different surface modifications and bead sizes are available for applications such as immobilization of proteins, purification of nucleic acids or coupling of a wide variety of molecules.

Hard Facts .

Applications .

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Accordion Content

Trace elements analysis

MagSi-S COOH beads with carboxyl surface are suitable for the preconcentration of metals in liquid samples. For analysis, the metals can be detached from the beads again by electrophoretic buffers.

Carpio, A., Mercader-Trejo, F., Arce, L., & Valcárcel, M. (2012). Use of carboxylic group functionalized magnetic nanoparticles for the preconcentration of metals in juice samples prior to the determination by capillary electrophoresis: CE and CEC. ELECTROPHORESIS, 33(15), 2446-2453. https://doi.org/10.1002/elps.201100636

Glycoprotein analysis

Lectins can be covalently coupled to MagSi-S Tosyl 1.0 beads to purify glycoproteins.

Engel, N. Y., Weiss, V. U., Wenz, C., Glück, S., Rüfer, A., Kratzmeier, M., Marchetti-Deschmann, M., & Allmaier, G. (2017). Microchip capillary gel electrophoresis combined with lectin affinity enrichment employing magnetic beads for glycoprotein analysis. Analytical and Bioanalytical Chemistry, 409(28), 6625-6634. https://doi.org/10.1007/s00216-017-0615-0

Immobilization of enzymes and peptides

Enzymes can also be immobilized on magnetic beads, e.g. for studies of kinetics or function experiments.

Huang, S., Celá, A., Adams, E., Glatz, Z., & Van Schepdael, A. (2020). Aldehyde oxidase assay by capillary electrophoresis: From off-line, online up to immobilized enzyme reactor. Journal of Separation Science, 43(17), 3565-3572. https://doi.org/10.1002/jssc.202000412

Coupling of oligonucleotides

Oligonucleotides can be covalently bound to the beads (e.g. MagSi-S NH2, MagSi-S CHO) for capture assays or specific enrichment of targets.

Detection of specific targets

Magnetic beads are also perfect for diagnostic purposes. They are often used for the detection of viruses, specific proteins, or cells.

Details of the MagSi-S Tools Beads .

Overview of functional surfaces .

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MagSi-S bead	Surface	Structure	Target molecule	Application

MagSi-S (Silica)	–	R-OH		Individual functionalization
MagSi-S CHO (Aldehyd)	Aldehyde	R-CHO	NH₂ (amine)	Protein immobilization
MagSi-S COOH (Carboxyl)	Carboxyl	R-COOH	NH₂ (amine); lysine, cysteine, histidine, tyrosine	Immobilization of peptides, proteins, antibodies
MagSi-S Epoxy	Epoxy		NH₂ (amine); lysine, cysteine, histidine, tyrosine	Immobilization of enzymes, peptides and proteins
MagSi-S Hydrazide	Hydrazide	R-CO-N₂H₂	Oligosaccharide groups	Immobilization of antibodies, peptides, proteins and glycoproteins
MagSi-S NH₂ (Amin)	NH₂	R-NH₂	Amine, aldehyde	Protein immobilization
MagSi-S SH (Sulfydryl)	SH	R-SH	Free cysteine	Immobilization of molecules via cysteine groups and immobilization on gold surfaces
MagSi-S Tosyl	Tosyl		SH (Sulfhydryl) and NH₂ (amine)	Immobilization of antibodies, peptides and proteins

Alle Beads sind in den Größen 600 nm, 1,0 µm und 3,0 µm erhältlich.

Coupling chemistries of different beads .

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Bead surface	Needed chemicals	Protein binding	Treatment	Comments
Carboxyl [1] (COOH)	EDC / NHS	Amine groups (of lysine and/or as unblocked N-termini) lysine, histidine, cysteine, tyrosine, etc.	No treatment necessary	Can be used for coupling for most proteins
Aldehyde (CHO)	Aldehyd/Amin reaction	Amine groups	No treatment necessary	Add reducing agent to stabilize the amide bond
Thiol (SH)	Redox reaction [3]	Free cysteine	Reduction of disulfides under non-denaturing conditions to generate free cysteine	Useful for cysteine-containing proteins. Multiple coupling possible
Amine [2] (NH₂)	Gluteraldehyde	Amine/Aldehyde	No treatment necessary	Add reducing agent to stabilize the amide bond
Tosyl	None	Sulfhydryl, amine groups	No treatment necessary	Useful for antibodies
Hydrazide	Sodium perodiate	Oligosaccharide groups	Oxidation of glycoproteins under non-denaturing conditions	Useful for glycoproteins
Epoxy	Adsorption / reaction support	Lysine, histidine, cysteine, tyrosine, etc.	No treatment necessary	Useful for enzymes

[1] In einem ersten Schritt werden die funktionellen Gruppen mit N-Hydroxysuccinimid aktiviert, so dass ein hochreaktiver Succinimidester entsteht, der mit den im Protein enthaltenen Aminogruppen reagiert.
[2] Gluteraldehyd führt zu einer stabileren Proteinbindung als die Carbodiimid-Reagenzien, die mit Carboxylat-Beads verwendet werden.
[3] Reduktion der Disulfide mit 0,1 M DTE (Dithioerythrol); Kopplung des Proteins bei einem pH-Wert unterhalb des isoelektrischen Punkts; Deaktivierung des überschüssigen Thiols mit 20 mM PDEA (2-(2-Pyridinyldithio)ethan-Amin)/ 1M NaCl, pH 4,3
Abkürzungen: EDC, N-Ethyl-N’-(dimethylaminopropyl)carbodiimid; NHS, N-Hydroxysuccinimid.

Technical data .

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	MagSi-S Tools 600	MagSi-S Tools 1.0	MagSi-S Tools 3.0
Size	600 nm	1.0 µm	3.0 µm
Concentration	10 mg/ml
Beads/ml	8 – 20 * 10^9	6 – 12 * 10^9	1 – 3 * 10^9
Product volume	2 ml, 10 ml, 100 ml
Material	Magentic silica beads with activated surface
Magnetic content	40%	60%	60%
Size distribution (D5 – D95)	500 – 900 nm	0,7 – 1,4 µm	0,6 – 10,0 µm
Buffer solution	MagSi-Tools, surface activated: PBS (pH 7.4), 0.05% sodium azide (NaN3, Toxic!), except: 1) MagSi-S, unmodified silica beads and MagSi-S NH2, amine-modified silica beads: Water, 0.05% sodium azide. 2) Epoxy and tosyl activated beads are supplied in DSMO:THF 1:1.
Storage	at 2 – 8°C

MagSi-S Beads .

Magnetic silica particles for proprietary development.

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Item number	Volume	Product	Concentration	Size
MD16003	2 ml	MagSi-S 600	10 mg/mL	600 nm
MD18003	10 ml
MD19003	100 ml
MD01003	2 ml	MagSi-S 1.0	10 mg/mL	1.0 µm
MD03003	10 ml
MD04003	100 ml
MD41003	2 ml	MagSi-S 3.0	10 mg/mL	3.0 µm
MD43003	10 ml
MD44003	100 ml

MagSi-S COOH Beads .

Magnetic silica particles with a carboxyl-modified surface. For carbodiimide coupling with NH2-containing molecules.

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Item number	Volume	Product	Concentration	Size
MD16004	2 mL	MagSi-S COOH 600	10 mg/mL	600 nm
MD18004	10 mL
MD19004	100 mL
MD01004	2 mL	MagSi-S COOH 1.0	10 mg/mL	1.0 µm
MD03004	10 mL
MD04004	100 mL
MD41004	2 mL	MagSi-S COOH 3.0	10 mg/mL	3.0 µm
MD43004	10 mL
MD44004	100 mL

MagSi-S Tosyl Beads .

Magnetic silica particles with tosyl modified surface. Intended for tosyl coupling chemistry with antibodies and proteins.

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Item number	Volume	Product	Concentration	Size
MD16008	2 mL	MagSi-S Tosyl 600	10 mg/mL	600 nm
MD18008	10 mL
MD19008	100 mL
MD01008	2 mL	MagSi-S Tosyl 1.0	10 mg/mL	1.0 µm
MD03008	10 mL
MD04008	100 mL
MD41008	2 mL	MagSi-S Tosyl 3.0	10 mg/mL	3.0 µm
MD43008	10 mL
MD44008	100 mL

MagSi-S NH2 Beads .

Magnetic silica particles with _{NH2 modified surface}. Intended for carbodiimide coupling chemistry with COOH-containing molecules or aldehyde coupling chemistry.

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Item number	Volume	Product	Concentration	Size
MD16005	2 ml	MagSi-S NH₂ 600	10 mg/mL	600 nm
MD18005	10 ml
MD19005	100 ml
MD01005	2 ml	MagSi-S NH₂ 1.0	10 mg/mL	1.0 µm
MD03005	10 ml
MD04005	100 ml
MD41005	2 ml	MagSi-S NH₂ 3.0	10 mg/mL	3.0 µm
MD43005	10 ml
MD44005	100 ml

MagSi-S SH Beads .

Magnetic silica particles with modified surface for SH coupling chemistry.

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Item number	Volume	Product	Concentration	Size
MD18006	10 ml	MagSi-S SH 600	10 mg/ml	600 nm
MD19006	100 ml
MD03006	10 ml	MagSi-S SH 1.0	10 mg/ml	1.0 µm
MD04006	100 ml
MD43006	10 ml	MagSi-S SH 3.0	10 mg/ml	3.0 µm
MD44006	100 ml

MagSi-S CHO Beads .

Magnetic silica particles with aldehyde modified surface. Intended for aldehyde coupling chemistry with molecules containing NH₂.

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Item number	Volume	Product	Concentration	Size
MD18007	10 ml	MagSi-S CHO 600	10 mg/ml	600 nm
MD19007	100 ml
MD03007	10 ml	MagSi-S CHO 1.0	10 mg/ml	1.0 µm
MD04007	100 ml
MD43007	10 ml	MagSi-S CHO 3.0	10 mg/ml	3.0 µm
MD44007	100 ml

MagSi-S Hydrazide Beads .

Magnetic silica particles with hydrazide modified surface. Intended for immobilization of antibodies, glycoproteins or other aldehyde containing molecules.

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Item number	Volume	Product	Concentration	Size
MD16013	2 ml	MagSi-S Hydrazide 600	10 mg/ml	600 nm
MD18013	10 ml
MD19013	100 ml
MD01013	2 ml	MagSi-S Hydrazide 1.0	10 mg/ml	1.0 µm
MD03013	10 ml
MD04013	100 ml
MD41013	2 ml	MagSi-S Hydrazide 3.0	10 mg/ml	3.0 µm
MD43013	10 ml
MD44013	100 ml

MagSi-S Epoxy Beads .

Magnetic silica particles with epoxy modified surface. Intended for coupling to enzymes and other NH₂-containing molecules.

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Item number	Volume	Product	Concentration	Size
MD16010	2 ml	MagSi-S Epoxy 600	10 mg/ml	600 nm
MD18010	10 ml
MD19010	100 ml
MD01010	2 ml	MagSi-S Epoxy 1.0	10 mg/ml	1.0 µm
MD03010	10 ml
MD04010	100 ml
MD41010	2 ml	MagSi-S Epoxy 3.0	10 mg/ml	3.0 µm
MD43010	10 ml
MD44010	100 ml

MagSi-S functional beads .

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