NGS and PCR cleanup .

Magnetic beads are very efficient for the purification of PCR products and size selection of amplicons in sequencing.

Our MagSi-NGSPREP Plus beads are comptible with most established NGS protocols, e.g. Ampure XP .

MagSi-DT Removal Beads remove dye terminators and unincorporated dyes from sequencing reactions – without centrifugation or filtration.

Hard Facts .

Compatible with existing protocols
High purity and recovery
Easily scalable and HTS compatible
MagSi DT-Removal: removal of impurities such as dye terminators
MagSi-NGSPREPplus: SPRI-based size selection
Guarantee consistent and robust results

Applications .

Library Preparation for NGS

Library preparation is one of the most important steps in sample preparation for NGS reactions. MagSi NGS beads allow easy size selection by varying the bead : sample ratio.

Size selection of PCR products

The MagSi-NGSPREP Plus beads are also suitable for size selection of your PCR products (SPRI).

By varying the bead-to-sample ratio, you can select desired fragment sizes or remove unwanted fragment sizes.

Purification of PCR products

With MagSi-NGSPREP Plus Beads you can remove enzymes, primers, oligonucleotides, as well as polymerases and salts easily and quickly from your PCR product.

Dye terminator removal

Removal of unincorporated dyes and salts after sequencing reactions with our MagSi-DT Removal Beads.

RNA enrichment

With an adapted protocol, MagSi-NGSPREP Plus beads can also be used to enrich RNA to improve the sensitivity and specificity of reverse transcription and qPCR analysis.

Simon, B., Pichon, M., Valette, M., Burfin, G., Richard, M., Lina, B., & Josset, L. (2019). Whole Genome Sequencing of A(H3N2) Influenza Viruses Reveals Variants Associated with Severity during the 2016-2017 Season. Viruses, 11(2), 108. https://doi.org/10.3390/v11020108

Details .

Easy-to-use .

MagSi-NGSPREP Plus .

Our MagSi-NGSPREP Plus beads are very efficient in NGS applications, both for size selection and library prep clean-up.

Excellent purification of PCR products
Efficient removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
Guaranteed consistent sequencing results
Protocol identical to Agencourt AMPure XP®.
Easy to automate, suitable for HTS
Optimized protocols for liquid handlers available (Biomek®, Hamilton® Microlab STAR)
Also suitable for liquid handlers from PerkinElmer®, Agilent Technologies® and others.
Best performance in combination with our magnetic devices for 96 and 384 well plates.

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Item number	Product	Volume
MDKT00010005	MagSi-NGSPREP Plus	5 ml
MDKT00010075	MagSi-NGSPREP Plus	75 ml
MDKT00010500	MagSi-NGSPREP Plus	500 ml

MagSi-NGSPREP Beads for best results .

Size selection of a DNA ladder using different bead to sample ratios (0.5x to 1.8x). Top on agarose gel, bottom with bioanalyzer measurements.

Source: magtivio

MagSi-DT Removal Beads .

MagSi-DT removal beads are very efficient in removing dye terminators from BigDye® sequencing reactions.

High signal intensity and Phred 20 read length
Efficient removal of unincorporated dye terminators and salts
Consistent and robust performance
Easy automation, HTS compatible
No centrifugation or filtration steps necessary
Purification directely in the reaction plates
Protocol identical to Agencourt CleanSEQ®.
Optimized for Biomek® Workstations and Hamilton® Microlab STARline
Compatible with many other automated liquid handling systems (e.g. PerkinElmer®, Caliper Life Sciences®, etc.)

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Item number	Product	Volume
MDKT00040008	MagSi-DT Removal	8 ml
MDKT00040050	MagSi-DT Removal	50 ml
MDKT00040500	MagSi-DT Removal	500 ml

MagSi-DT Removal Beads for best results .

The MagSi-DT Removal Beads are very efficient in removing dye terminators from BigDye® sequencing reactions.

NGS and DT Removal Beads .

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