Protein purification .

Magnetic beads are ideal for purification and isolation of proteins and peptides for bioassays, mass spectrometry, SDS-PAGE, immunoassays and biomarker analysis.

MagSi Protein A and Protein G beads bind the Fc region of antibodies and can be used for immunoprecipitation or immunoassays.

MagSi-S proteomics beads with C4, C8, or C18 surface modification enable the purification or desalting of large biomolecules such as proteins as well as smaller peptides.

MagSi-WCX and MagSi-WAX can be used for the enrichment and desalting of proteins and peptides.

Hard Facts .

Fast and easy protein purification
High purity and yield
Wide range of applications
Different coatings available
Alkyl radicals (C4-C18) for reverse phase applications
Weak Cation / Anion Exchange (WCX / WAX) available

Applications .

Accordion title

Accordion Content

Protein preparation for mass spectrometry

MagSi beads are suitable for sample preparation for analyses such as MALDI-TOF. Low molecular weight peptides from plasma or urine can be concentrated and used directly in MS after elution.
Hussien, A. K., Moez, P. E., Elwakil, H. S., & Elagaan, H. A. (2020). Identification of urinary proteomic profile of patients with chronic allograft nephropathy. Alexandria Journal of Medicine, 56(1), 93-104. https://doi.org/10.1080/20905068.2020.1749782

Rizk, M. M., Sharaki, O. A., Meleis, M. E., Younan, D. N., Elkial, A. A., & Moez, P. (2019). Detection of Epithelial Ovarian Cancer using C8Magnetic Bead Separation and MALDI-TOF Plasma Proteome Profiling in Egyptian Females. Asian Pacific Journal of Cancer Prevention, 20(12), 3603-3609. https://doi.org/10.31557/APJCP.2019.20.12.3603

Protein preparation for SDS-PAGE analyses

Purification of a wide variety of proteins for size analysis on SDS-PAGEs.

Separation of peptides and proteins

Separation of peptides and proteins for downstream applications.

Detergent removal

Removal of detergents from protein and peptide solutions.

Enrichment of phosphorylated proteins and peptides

Enrichment of phosphorylated proteins and peptides with the MagSi-WCX beads.

Biomarker analysis

Biomarker analyses can be supported by proteome purification via magnetic beads.

Immunoprecipitation

Antigens can be precipitated by incubation with specific antibodies. These immunoprecipitates can be fished from the samples by beads containing covalently bound protein A or protein G.

Hjeij, R., et al. (2014). CCDC151 Mutations Cause Primary Ciliary Dyskinesia by Disruption of the Outer Dynein Arm Docking Complex Formation. The American Journal of Human Genetics, 95(3), 257-274. https://doi.org/10.1016/j.ajhg.2014.08.005

IgG purification

Magentic beads with covalently bound protein A or protein G are excellent for binding and purification of IgG antibodies from samples.

Details .

Product overview protein and peptide purification .

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	Surface modification	Concentration	Target molecule
MagSi-Protein A	Protein A	10 mg/ml	Immunoglobulin (Fc region)
MagSi-Protein G	Protein G	10 mg/ml	Immunoglobulin (Fc region)
MagSi-proteomics	Alkyl chains	10 mg/ml	Peptides and proteins
MagSi-WCX	Weak Cation Exchange Surface	20 mg/ml	Proteins with positive net charge
MagSi-WAX	Weak Anion Exchange Surface	20 mg/ml	Proteins with negative net charge

Alle Beads sind in den Größen 600 nm, 1,0 µm und 3,0 µm erhältlich.

MagSi Protein A Beads .

Magnetic beads with covalently bound recombinant protein A for antibody purification and immunoassays.

Efficient binding of the Fc region of immunoglobulins.
Simple immobilization of various biomolecules
Different types and particle sizes, ideal for immunoprecipitation (IP) and IgG purification
Also suitable for HTS due to simple automation
3 sizes available: 600 nm, 1000 nm or 3000 nm diameter

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Item number	Volume	Product	Concentration	Size
MD10011	1 ml	MagSi-protein A 600	10 mg/ml	600 nm
MD11011	5 ml
MD01011	1 ml	MagSi-protein A 1.0	10 mg/ml	1.0 µm
MD02011	5 ml
MD41011	1 ml	MagSi-protein A 3.0	10 mg/ml	3.0 µm
MD42011	5 ml

Binding strength to protein A .

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Species	Antibody class	Protein A binding
Human	Total IgG	++++
	IgG1	+
	IgG2	++++
	IgG3	++++
	IgG4	++++
Mouse	Total IgG	++++
	IgG1	+
	IgG2A	++++
	IgG2B	++++
	IgG3	+++
Rat	Total IgG	+
	IgG1	–
	IgG2A	–
	IgG2B	–
	IgG2C	++
Hamster	Total IgG	++
Guinea pig	Total IgG	++++
Rabbit	Total IgG	++++
Horse	Total IgG	++
Cow	Total IgG	++
Pig	Total IgG	+++
Sheep	Total IgG	+
Goat	Total IgG	+
Chicken	Total IgG	–

Quelle: magtivio

MagSi Protein G Beads .

Magnetic beads with covalently bound protein G for antibody purification and immunoassays

Recombinant protein G covalently bound to the bead surface
Efficient binding of the Fc region of immunoglobulins.
Suitable for immobilization of different biomolecules
Different particle sizes, ideal for immunoprecipitation (IP) and IgG purification
Suitable for HTS due to simple automation capability
3 bead sizes available: 600 nm, 1000 nm or 3000 nm diameter

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Item number	Volume	Product	Concentration	Size
MD10012	1 ml	MagSi-protein G 600	10 mg/ml	600 nm
MD11012	5 ml
MD01012	1 ml	MagSi-protein G 1.0	10 mg/ml	1.0 µm
MD02012	5 ml
MD41012	1 ml	MagSi-protein G 3.0	10 mg/ml	3.0 µm
MD42012	5 ml

Binding strength to protein G .

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Species	Antibody class	Protein G binding
Human	Total IgG	++++
	IgG1	++++
	IgG2	++++
	IgG3	++++
	IgG4	++++
Mouse	Total IgG	++++
	IgG1	+++
	IgG2A	++++
	IgG2B	++++
	IgG3	+++
Rat	Total IgG	++
	IgG1	+
	IgG2A	++++
	IgG2B	++
	IgG2C	+++
Hamster	Total IgG	++
Guinea pig	Total IgG	++
Rabbit	Total IgG	+++
Horse	Total IgG	++++
Cow	Total IgG	++++
Pig	Total IgG	++
Sheep	Total IgG	++
Goat	Total IgG	++
Chicken	Total IgG	–

Quelle: magtivio

MagSi-proteomics Beads .

MagSi-S proteomics beads are optimized for purification, concentration or desalting of peptides and protein fragments. The surface of MagSi proteomics beads is modified by covalently bonded C4, C8 or C18 alkyl groups and is therefore very well suited for reverse phase applications.

MagSi-S proteomics C4: suitable for capture, concentration and purification of proteins from protein mixtures, cell lysates, culture supernatant (e.g. secreted proteins).

MagSi-S proteomics C8: suitable for the capture and purification of peptides and proteins from clinical samples such as urine, saliva and cerebrospinal fluid.

MagSi-S proteomics C18: suitable for desalting of peptides or trypsin protein digestion prior to mass spectrometry, concentration of peptides (e.g. secreted peptides), capture and purification of peptides and proteins from serum and plasma.

Beads for purification, concentration and desalting of peptides or protein digestion
Also suitable for sample preparation before mass spectrometry (LC-MS/MS)
Surface-bound alkyl groups (C4, C8 or C18) for reverse phase applications
Easy to automate and suitable for HTS

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Item number	Product	Surface modification	Volume
MD01014	MagSi-S proteomics C4	C4 alkyl group	2 ml
MD02014			10 ml
MD03014			100 ml
MD01015	MagSi-S proteomics C8	C8 alkyl group	2 ml
MD02015			10 ml
MD03015			100 ml
MD01009	MagSi-S proteomics C18	C18 alkyl group	2 ml
MD03009			10 ml
MD04009			100 ml

MagSi-WCX (weak cation exchange) Beads .

Magnetic silica particles with weak cation exchange surface (WCX). MagSi-WCX is ideal for reducing the complexity of proteins or peptides. Applications include sample preparation and pre-fractionation prior to mass spectrometry or SDS-PAGE analysis, biomarker analysis, and serum/plasma profiling.

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Item number	Product	Volume
MD01023	MagSi-WCX	2 ml
MD02023		10 ml
MD03023		100 ml

MagSi-WAX (weak anoin exchange) Beads .

Magnetic silica particles with weak anion exchange surface (WAX). MagSi-WAX is ideal for reducing the complexity of proteins or peptides. Applications include sample preparation and pre-fractionation prior to mass spectrometry or SDS-PAGE analysis, biomarker analysis, and serum/plasma profiling.

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Item number	Product	Volume
MD01025	MagSi-WAX	2 ml
MD02025		10 ml
MD03025		100 ml

Magnetic beads for protein purification .

Magentic beads available with different surfaces: Protein A or G coupling, alkyl residues as well as WCX or WAX beads.

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Downloads .

Manuals and product information

Publications

Manuals and product information

Publications

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Steinbrenner Laborsysteme GmbH

Protein purification .

Hard Facts .

Applications .

Details .

Product overview protein and peptide purification .

Simple workflow for protein purification .

MagSi Protein A Beads .

Binding strength to protein A .

MagSi Protein G Beads .

Binding strength to protein G .

MagSi-proteomics Beads .

MagSi-WCX (weak cation exchange) Beads .

MagSi-WAX (weak anoin exchange) Beads .

Magnetic beads for protein purification .

Downloads .

IgG Crosslinking zu MagSi-Protein A/G – Technische Information 116.29 KB

MagSi-Protein A Beads – Produktdatenblatt 173.99 KB

MagSi-Protein G Beads – Produktdatenblatt 115.00 KB

MagSi-proteomics – Produktdatenblatt 233.85 KB

MagSi-WAX – Produktdatenblatt 249.69 KB

MagSi-WCX – Produktdatenblatt 249.32 KB

Peptid- und Proteinaufreinigung mit MagSi-Beads – Selection Guide 1.77 MB