MagSi-STA - Bead meets Streptavidin .

Magnetic particles are ideal as a solid support phase for immunoassays. They enable simplified workflows and automated processes.

Our superparamagnetic MagSi-STA silica beads are coated with a modified streptavidin. This makes them ideal for purification or capture of biotinylated molecules.

Key Facts .

Streptavidin-coupled magnetic beads.
High binding capacity of up to 6800 pmol biotin/mg beads
Simple handling
Short and fast protocol
Low non-specific interactions
Different bead sizes available

Applications .

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Accordion Content

Capture of biotinylated molecules

MagSi-STA beads are perfect for capturing biotinylated molecules. Molecules bound to the beads can be removed or purified from the sample using simple protocols.

Immunoassays

The MagSi-STA beads are ideally suited as solid support phase of immunoassays. In “sandwich assays”, e.g. antigens or biotinylated antibodies can be detected very specifically.

Schmalenberg, M., Beaudoin, C., Bulst, L., Steubl, D., & Luppa, P. B. (2015). Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care. Journal of Immunological Methods, 427, 36-41. https://doi.org/10.1016/j.jim.2015.09.004

RNA depletion

When gene expression profiling using RNA-Seq, interfering rRNA must be removed. For this “depletion”, one uses sequence-specific biotinylated oligonucleotides that bind to strepdavidin beads such as MagSi-STA 600 BI. These can then be easily removed from the reaction mixture by magnet.

Immunoprecipitation

Antigens can also be elegantly and very specifically captured with biotinylated antibodies. The resulting antigen-antibody complexes bind to streptavidin-coupled beads and can then be easily and rapidly purified or concentrated.

Capture from cells

The fishing of specific cells for further cell line analysis is greatly simplified by Magnetic Beads. This involves mixing cells with cell line-specific biotinylated antibodies and then fishing them with streptavidin-coupled MagSi-STA.

Wirth, F., Huck, K., Lubosch, A., Zoeller, C., Ghura, H., Porubsky, S., & Nakchbandi, I. A. (2021). Cdc42 in osterix-expressing cells alters osteoblast behavior and myeloid lineage commitment. Bone, 153, 116150. https://doi.org/10.1016/j.bone.2021.116150

Details .

Magnetic beads as solid phase in immunoassays .

Sandwich assay for the detection of antigens:

Biotinylated antibody binds to bead (solid phase)
Target protein / antigen binds to antibody and is thus fished out of solution
Secondary antibody with reporter (HRP) binds to target/antigen. This complex is detected via the reporter’s chemiluminescent signal.

MagSi-STA .

MagSi-STA beads are available in two types with different streptavidin coupling chemistry.

Carboxyl (C) coupled beads – with additional spacer – are suitable for applications that require a more hydrophilic surface.

Tosyl (T) coupled beads are optimized for applications that require a more hydrophobic surface.

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Product	Coupling chemistry	Concentration	Size	Binding capacity (pmol biotin/mg beads)
MagSi-STA 600	Carboxyl	10 mg/ml	600 nm	3500-5000
MagSi-STA 600 BI	Carboxyl	10 mg/ml	600 nm	6000-6800
MagSi-STA 1.0	Carboxyl	10 mg/ml	1,0 µm	3500-5000
MagSi-STA 1.0 L	Carboxyl	10 mg/ml	1,0 µm	1200-2000
MagSi-STA 3.0 L	Carboxyl	10 mg/ml	3,0 µm	700-1200
MagSi-STA 1.0 TL	Tosyl	10 mg/ml	1,0 µm	1200-2000
MagSi-STA 1.0 TS	Tosyl	10 mg/ml	1,0 µm	3500-5000
MagSi-STA 3.0 TL	Tosyl	10 mg/ml	3,0 µm	500-900

Capture and depletion of biotinylated proteins .

Capturing biotinylated molecules using MagSi-STA Magnetic Beads is super simple. The protocol consists of only three steps: bind, wash, elute.

The MagSi-STA beads with surface-bound streptavidin are added to the solution containing the biotinylated analyte. After a short incubation, the beads are collected by magnets, washed several times and resuspended.

MagSi-STA Beads with Carboxyl Coupling .

MagSi-STA beads are available in two different types – depending on the streptavidin coupling chemistry. Carboxyl-coupled beads – with additional spacer- are suitable for applications that require a more hydrophilic surface.

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Item number	Volume	Product	Concentration	Size	Binding capacity (pmol biotin/mg beads)
MD16001	2 ml	MagSi-STA 600	10 mg/ml	600 nm	3500-5000
MD18001	10 ml
MD19001	100 ml
MD21001	2 ml	MagSi-STA 600 BI	10 mg/ml	600 nm	6000-6800
MD31001	10 ml
MD41001	100 ml
MD01001	2 ml	MagSi-STA 1.0	10 mg/ml	1.0 µm	3500-5000
MD03001	10 ml
MD04001	100 ml
MD06001	2 ml	MagSi-STA 1.0 L	10 mg/ml	1.0 µm	1200-2000
MD07001	10 ml
MD08001	100 ml
MD33001	2 ml	MagSi-STA 3.0 L	10 mg/ml	3.0 µm	700-1200
MD34001	10 ml
MD35001	100 ml

MagSi-STA Beads with tosyl coupling .

MagSi-STA tosyl beads are optimal for applications that require a hydrophobic surface.

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Item number	Volume	Product	Concentration	Size	Binding capacity (pmol biotin/mg beads)
MD25001	2 ml	MagSi-STA 1.0 TL	10 mg/ml	1.0 µm	1200-2000
MD26001	10 ml
MD27001	100 ml
MD29001	2 ml	MagSi-STA 1.0 TS	10 mg/ml	1.0 µm	3500-5000
MD30001	10 ml
MD31001	100 ml
MD37001	2 ml	MagSi-STA 3.0 TL	10 mg/ml	3.0 µm	500-900
MD38001	10 ml
MD39001	100 ml

MagSi-STA Beads .

Available in 600 nm, 1.0 µm and 3.0 µm sizes with carboxyl or tosyl surface.

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Downloads .

Manuals

MagSi-STA Trial Kit – Produktinformation und Manual 261.67 KB

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MagSi-STA – Produktinformation und Manual 261.07 KB

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Publications

Martin, J. G., Gupta, M., Xu, Y., Akella, S., Liu, J., Dordick, J. S., & Linhardt, R. J. (2009). Toward an artificial Golgi: redesigning the biological activities of heparan sulfate on a digital microfluidic chip. Journal of the American Chemical Society, 131(31), 11041-11048. https://doi.org/10.1021/ja903038d

Chicher, J., Simonetti, A., Kuhn, L., Schaeffer, L., Hammann, P., Eriani, G., & Martin, F. (2015). Purification of mRNA-programmed translation initiation complexes suitable for mass spectrometry analysis. Proteomics, 15(14), 2417-2425. https://doi.org/10.1002/pmic.201400628

Martin, F., Ménétret, J.-F., Simonetti, A., Myasnikov, A. G., Vicens, Q., Prongidi-Fix, L., Natchiar, S. K., Klaholz, B. P., & Eriani, G. (2016). Ribosomal 18S rRNA base pairs with mRNA during eukaryotic translation initiation. Nature Communications, 7, 12622. https://doi.org/10.1038/ncomms12622

Shah, T., Qin, S., Vashi, M., Predescu, D. N., Jeganathan, N., Bardita, C., Ganesh, B., diBartolo, S., Fogg, L. F., Balk, R. A., & Predescu, S. A. (2018). Alk5/Runx1 signaling mediated by extracellular vesicles promotes vascular repair in acute respiratory distress syndrome. Clinical and Translational Medicine, 7(1), 19. https://doi.org/10.1186/s40169-018-0197-2

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