Quantitative PCR

Only the best for your qPCR.

Our primaQUANT Master Mixes for SYBR® Green- or probe-based qPCR guarantee reliable and reproducible results. They are characterized by high sensitivity, great robustness and excellent sensitivity. The detection limit is below 10 pg DNA.
The primaQUANT qPCR Master Mixes, like all our molecular biology reagents, are developed and produced entirely in Germany.

Many possibilities .

Genotyping

RNA / miRNA expression

Diagnostics

Quality control

1-Step and 2-Step

probes or SYBR® Green

HotStart

Ready-to-use

Features .

Excellent performance .

The primaQUANT ADVANCED qPCR Master Mixes with SYBR Green offer a wide dynamic range and excellent performance, even with low template quantities or targets with low expression.

For all qPCR cyclers .

Our qPCR 2x Master Mixes are suitable for all common qPCR cyclers and optionally available with ROX.

We have compiled a table for you showing whether your device uses ROX(6-carboxy- X-rhodamine) for normalization and which concentration, if any, is required.

Save as
csv
xlsx
pdf
print

Manufacturer	qPCR cycler	ROX necessary?
Applied Biosystems / Thermo Fischer	StepOne™ Real-Time PCR System	high ROX
	StepOnePlus™ Real-Time PCR System	high ROX
	7500 Real-Time PCR System	low ROX
	7500 Fast Real-Time PCR System	low ROX
	7500 Fast Dx Real-Time PCR Instrument	low ROX
	7500 Real-Time PCR System for Human Identification	low ROX
	7300 Real-Time PCR System	high ROX
	Viia™ 7 Real-Time PCR System	low ROX
	7900HT Fast Real-Time PCR System	high ROX
	OpenArray® Real-Time PCR Platform	high ROX
	PRISM® 7000 Sequencing Detection System	high ROX
	PRISM® 7700 Sequencing Detection System	high ROX
	PRISM® 7900 Sequencing Detection System	high ROX
	Gene Amp 5700	high ROX
	Quantstudio™ 3 / 3 FAST	low Rox
	Quantstudio™ 5 / 5 FAST	low Rox
	Quantstudio™ 6	low Rox
	Quantstudio™ 7	low Rox
	Quantstudio™ 12	low Rox
	QuantStudio™ 12K Flex system	low ROX
Bioneer	Exicycler™96	no ROX, optional
	Exicycler™384	no ROX, optional
Biorad	CFX96™ Real-Time PCR Detection System	no ROX
	CFX96 Touch™ Real-Time PCR Detection System	no ROX
	CFX384 Touch™ Real-Time PCR Detection System	no ROX
	CFX Opus 96 Real-Time PCR System	no ROX
	CFX Opus 384 Real-Time PCR System	no ROX
	CFX Connect™ Real-Time PCR Detection System	no ROX
	iQ5 Real-Time PCR Detection System	no ROX
	MiniOpticon™ Real-Time PCR Detection System	no ROX
	Opticon 2 – Continuous Fluorescence Detection System	no ROX
	Chromo4™ Four-Color Real-Time Detector	no ROX
Bioron	RealLine Cycler 48-4	no ROX, optional
	RealLine Cycler 48-5	no ROX, optional
	RealLine Cycler 96-4	no ROX, optional
	RealLine Cycler 96-5	no ROX, optional
DNA Technology	Dtlite	no ROX, optional
	Dtprime	no ROX, optional
Eppendorf	Mastercycler® ep realplex 4, Real-Time Thermal Cycler	no ROX
	Mastercycler® ep realplex 4s, Real-Time Thermal Cycler	no ROX
	Mastercycler® ep realplex, Real-Time Thermal Cycler	no ROX
	Mastercycler® ep realplex s, Real-Time Thermal Cycler	no ROX
	Mastercycler Nexus	no ROX
	Mastercycler Nexus gradient	no ROX
	Mastercycler Pro	no ROX
	Mastercycler Pro S	no ROX
	Mastercycler Pro 384	no ROX
Roche	LightCycler® 96 System	no ROX
	LightCycler® 480 System	no ROX
	LightCycler® 2.0 Instrument	no ROX
	LightCycler® 1.5 Instrument	no ROX
	LightCycler® 1536 System	no ROX
	LightCycler® Nano System	no ROX
Analytik Jena	qTower	low ROX (optional)
	qTower 2.0	low ROX (optional)
	qTower 2.2	low ROX (optional)
	qTower 3	low ROX (optional)
	qTower 3 84	low ROX (optional)
Stratagene / Agilent	AriaMx Real-time PCR System	no ROX
	Mx3000P® qPCR System	low ROX
	Mx3005P® qPCR System	low ROX
	Mx4000® qPCR System	low ROX
Qiagen	Rotor-Gene™ Q	low ROX
	Rotor-Gene™ 6000	low ROX
Quantabio	Q	no ROX, optional

qPCR in less than 20 minutes .

Extremely fast

The primaQUANT qPCR Master Mixes cope with the shortest cycle times due to their ultra fast DNA polymerase: a complete qPCR run takes less than 20 minutes under suitable conditions.

Of course, you can also use your previous qPCR protocol.

Standard protocol:

Save as
xlsx
pdf
print

	Schritt	Zeit	Temperatur
	Initiale Denaturierung	1-3 Minuten	92°C – 95°C
25 – 40 Zyklen	Denaturierung	5-10 Sekunden	92°C – 95°C
	Kombiniertes Annealing/Elongation	10-20 Sekunden	60°C – abhängig von den verwendeten Primern

Fast protocol:

Save as
xlsx
pdf
print

	Schritt	Zeit	Temperatur
	Initiale Denaturierung	1 Minute	92°C – 95°C
25 – 40 Zyklen	Denaturierung	5 Sekunden	92°C – 95°C
	Kombiniertes Annealing/Elongation	10 Sekunden	60°C – abhängig von den verwendeten Primern

Ultra Fast Protocol:

Save as
xlsx
pdf
print

	Schritt	Zeit	Temperatur
	Initiale Denaturierung	1 Minute	92°C – 95°C
25 – 40 Zyklen	Denaturierung	1 Sekunde	92°C – 95°C
	Kombiniertes Annealing/Elongation	1-5 Sekunden	60°C – abhängig von den verwendeten Primern

Temperature-stable .

Freeze/thaw

Our PCR and qPCR reagents are not affected by multiple freezing/thawing . You can therefore freeze and thaw the mixes more than 10 times without any problems.

Storage in the refrigerator

The primaQUANT Master Mixes are stable for weeks at 4°C. For short-term storage, we generally recommend storage at 4°C in the refrigerator.

Stability at room temperature

The primaQUANT Master Mixes can even be stored for several days at room temperature without loosing quality. Therefore, you no longer have to pipette your qPCR laboriously on ice to avoid a loss of sensitivity!

Smart Package Size .

No more annoying aliquoting!

Our Master Mixes come in units of 1 ml as standard.

This is very convenient and practical, because one tube is enough for exactly one 96 well plate, two tubes for one 384 well plate. If you ever need less, you can simply store the mixes in the refrigerator for several weeks.

Customized fillings: As the manufacturer, we can of course supply you with fillings and mixtures according to your special requirements.

Made in Germany .

We develop and produce our enzymes and master mixes exclusively ourselves in Germany. You benefit from this in several ways:

Our qPCR product groups .

For most applications we can offer the right product – just ask us.

qPCR (SYBR® Green)

Advanced Master Mixes with SYBR® Green for qPCR

qPCR (Probe)

Advanced Master Mixes for probe-based qPCR, e.g. Taqman®.

qPCR (SYBR® Green)

Master Mixes with SYBR® Green for qPCR

qPCR (Probe)

Master Mixes for probe-based qPCR, e.g. Taqman®.

1-Step RT-qPCR for probes

Master mixes for one-step RT-qPCR without separate rewrite of mRNA.

1-Step RT-qPCR with SYBR® Green

Master mixes for one-step RT-qPCR without separate rewrite of mRNA.

Would you like more information or a demo?

Then please feel free to contact us.
We look forward to your call at +49 (0) 6223 96 73 00 or an email.

You want to transcribe RNA into cDNA ?

For the determination of RNA expression, detection of RNA viruses or RNA-seq, the (m)RNA to be detected must first be transcribed into cDNA.
With our primaREVERSE RT kit, you synthesize cDNA with high efficiency and in the shortest possible time for your downstream applications.

Extremely fast: cDNA in under 15 minutes
High yield due to reduced RNase-H activity
Ready-to-use: complete kit incl. Primer and DNase
For templates up to 17 kb in length
Also for complex transcripts

Sie haben Fragen?

Steinbrenner Laborsysteme GmbH

Quantitative PCR

Only the best for your qPCR.

Many possibilities .

Genotyping

RNA / miRNA expression

Diagnostics

Quality control

1-Step and 2-Step

probes or SYBR® Green

HotStart

Ready-to-use

Features .

Excellent performance .

For all qPCR cyclers .

qPCR in less than 20 minutes .

Temperature-stable .

Smart Package Size .

Made in Germany .

Our qPCR product groups .

qPCR (SYBR® Green)

qPCR (Probe)

qPCR (SYBR® Green)

qPCR (Probe)

1-Step RT-qPCR for probes

1-Step RT-qPCR with SYBR® Green

Would you like more information or a demo?

You want to transcribe RNA into cDNA ?