qPCR with probes .

Our primaQUANT Probe qPCR Master Mixes are suitable for all common types of probe assays:

• Primer probes
• Hydrolysis probes (TaqMan, MGB-TaqMan, Snake Assay)
• Hybridization probes (Hybprobe or FRET, Molecular Beacons, HyBeacon, MGB-Pleiades, MGB-Eclipse, ResonSense, Yin-Yang or displacing);
• Nucleotide analogs (PNA, LNA, ZNA, non-natural bases: Plexor primer, Tiny-Molecular Beacon).

More information can be found in the following review:

Navarro, E., Serrano-Heras, G., Castaño, M. J., & Solera, J. (2015). Real-time PCR detection chemistry. Clinica Chimica Acta, 439, 231-250. https://doi.org/10.1016/j.cca.2014.10.017

The primaQUANT Probe qPCR Master Mix is perfect for genotyping using probes. Multiplexing allows several markers to be detected in parallel.

Matsuda, K. (2017). PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement. Advances in Clinical Chemistry, 80, 45-72. https://doi.org/10.1016/bs.acc.2016.11.002

To determine the expression of a gene (gene or RNA expression), the total RNA must first be transcribed into cDNA.

If you want to perform cDNA synthesis in a separate step, our primaREVERSE RT kit first choice.

Alternatively, you can also transfer the RNA directly into our primaQUANT 1STEP RT-qPCR Kit.

Multiplex qPCR assays use multiple target-specific probes in parallel, each labeled with a different fluorescent dye. In this way, the expression values of several targets or genes can be determined in parallel in a time-saving manner. This elegant method is widely used, especially in diagnostics.

Hawkins, S. F. C., & Guest, P. C. (2017). Multiplex Analyses Using Real-Time Quantitative PCR. In P. C. Guest (Ed.), Multiplex biomarker techniques: methods and applications (pp. 125-133). Springer. https://doi.org/10.1007/978-1-4939-6730-8_8

Quantitative PCR is also frequently used for the diagnosis of pathogens.

DNA pathogens (DNA viruses, bacteria, fungi) can thus be analyzed directly. For RNA viruses, e.g. SARS-COV2, a transcription of RNA into cDNA is necessary. Our primaREVERSE RT cDNA Synthesis Kit is ideally suited for this purpose. You can also use our primaQUANT 1-STEP Master Mix to perform cDNA synthesis and qPCR in one step.

Because of their high purity, our primaQUANT Master Mixes are ideal for detecting or ruling out contamination of a product with nucleic acids or nucleases.