Pathogen DNA and RNA Extraction .
The MagSi-NA Pathogens and MagSi-DX Pathogen Plus kits enable the reliable purification of DNA and RNA with high purity and yield. The nucleic acids obtained are ideal for pathogen detection using, for example, qPCR or NGS.
The MagSi-DX Pathogen Plus Kit has been specifically validated for the extraction of viral and bacterial nucleic acids from human clinical samples such as whole blood, serum, plasma, urine, stool, swabs, and saliva. It enables both manual and automated purification and is compatible with standard liquid-handling systems.
While MagSi-NA Pathogens is designed for research applications, MagSi-DX Pathogen Plus is CE-IVD certified and is therefore intended for use in diagnostic workflows.
Key Facts .
- Consistently high-quality DNA/RNA yield
- Time required for 96 samples <30 min
- No cooling, centrifugation or filtration required
- Compatible with Kingfisher™ and other automation solutions
- Optimized for lysates with high viscosity
- Suitable for many downstream applications
- CE-IVD certified (MagSi-DX Pathogen Plus)
Applications .
The CE-IVD certified MagSi-DX Pathogen Kit is recommended for the manual and automated purification of RNA for the diagnosis of human samples (respiratory swabs, saliva). The purified RNA is suitable for all common methods for the detection of viral SARS-CoV-2 RNA, especially RT-qPCR and sequencing.
Jonckheere, I., Faes, L., Overmeire, Y., De Vleeschauwer, A., Vanden Daele, L., Van Bruaene, N., Vandecandelaere, I., Merlaen, B., van Cann, J., & Vandesompele, J. (2021). Equivalence of saliva RT-qPCR testing to nasal-throat/nasopharyngeal swab testing in the general practitioner’s setting to detect SARS-CoV-2 [Preprint]. Infectious Diseases (except HIV/AIDS). https://doi.org/10.1101/2021.09.30.21264181
Manual and automated purification of RNA for virus detection by PCR, qPCR and LAMP.
The MagSi-NA and the CE-IVD certified MagSi-DX Pathogen Kit enable cost-effective extraction of DNA and RNA from a variety of sample materials: serum, plasma, oropharyngeal and nasopharyngeal swabs or other respiratory samples. The isolated nucleic acids can be used for qPCR or other common pathogen detection methods.
MagSi-DX Pathogen Plus / MagSi-NA Pathogen .
Simple workflow, fast and cost-effective .
The purification of RNA and DNA using MagSi beads is very simple and fast.
The protocol consists of only four steps: lysis, NA binding, washing, eluting.
The lysed sample material is mixed with the Magnetic Beads. The nucleic acids bind to the beads, which can be easily separated and washed using a magnet. In the final step, the bound DNA is released from the beads again using the elution buffer.
The MagSi-NA and the MagSi-DX Pathogen Kit can be used with Kingfisher™, PurePrep™, Hamilton® and TECAN® systems, among others.
Kits for DNA/RNA extraction from pathogens .
All kits are available in different package sizes.
